Proteins involved in oxidative stress in leiomyoma tissues treated with ulipristal acetate

Blendi Ura, Lorenzo Monasta, Yeraldin Chiquinquira Castillo de Spelorzi, Giorgio Arrigoni, Cinzia Franchin, Stefania Biffi, Michelangelo Aloisio, Bartolomea Gaita, Danilo Licastro, Emmanouil Athanasakis, Federica Scrimin, Guglielmo Stabile, Federico Romano, Giovanni Di Lorenzo, Giuseppe Ricci

Research output: Contribution to journalArticlepeer-review

Abstract

Uterine leiomyoma presents the highest incidence among benign tumors of the female reproductive tract. The present study compared the proteome of leiomyoma treated with ulipristal acetate with that of untreated leiomyoma to investigate protein expression patterns in relation to oxidative stress. Paired tissue samples from seven treated and untreated leiomyomas were collected and the proteome was analyzed by two‑dimensional gel electrophoresis (2‑DE). Western blotting was used to validate the results of 2‑DE, and mass spectrom‑ etry was used to identify proteins. The tissue expression of 30 proteins was markedly affected by treatment with ulipristal acetate. Bioinformatics analysis revealed that several of the differentially expressed proteins were involved in the degrada‑ tion of hydrogen peroxide and the synthesis of reactive oxygen species. The present study suggested the involvement of oxida‑ tive stress as a novel mechanism of action of ulipristal acetate. These findings require further investigations to understand the role of ulipristal acetate in the treatment of the leiomyoma.

Original languageEnglish
Article number11642
JournalMolecular Medicine Reports
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 2021

Keywords

  • Leiomyoma
  • Mass spectrometry
  • Proteomics
  • Two‑dimensional electrophoresis
  • Ulipristal acetate

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

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