Proteins modulation in human skeletal muscle in the early phase of adaptation to hypobaric hypoxia

Agnese Viganò, Marilena Ripamonti, Sara De Palma, Daniele Capitanio, Michele Vasso, Robin Wait, Carsten Lundby, Paolo Cerretelli, Cecilia Gelfi

Research output: Contribution to journalArticlepeer-review


High altitude hypoxia is a paraphysiological condition triggering redox status disturbances of cell organization leading, via oxidative stress, to proteins, lipids, and DNA damage. In man, skeletal muscle, after prolonged exposure to hypoxia, undergoes mass reduction and alterations at the cellular level featuring a reduction of mitochondrial volume density, accumulation of lipofuscin, a product of lipid peroxidation, and dysregulation of enzymes whose time course is unknown. The effects of 7-9 days exposure to 4559 m (Margherita Hut, Monte Rosa, Italy) on the muscle proteins pattern were investigated, pre- and post-exposure, in ten young subjects, by 2-D DIGE and MS. Ten milligram biopsies were obtained from the mid part of the vastus lateralis muscle at sea level (control) and at altitude, after 7-9 days hypoxia. Differential analysis indicates that proteins involved in iron transport, tricarboxylic acid (TCA) cycle, oxidative phosphorylation, and oxidative stress responses were significantly (p

Original languageEnglish
Pages (from-to)4668-4679
Number of pages12
Issue number22
Publication statusPublished - Nov 2008


  • 2-D DIGE
  • Hypoxia
  • Mass spectrometry
  • Muscle

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry


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