Proteins modulation in human skeletal muscle in the early phase of adaptation to hypobaric hypoxia

Agnese Viganò, Marilena Ripamonti, Sara De Palma, Daniele Capitanio, Michele Vasso, Robin Wait, Carsten Lundby, Paolo Cerretelli, Cecilia Gelfi

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

High altitude hypoxia is a paraphysiological condition triggering redox status disturbances of cell organization leading, via oxidative stress, to proteins, lipids, and DNA damage. In man, skeletal muscle, after prolonged exposure to hypoxia, undergoes mass reduction and alterations at the cellular level featuring a reduction of mitochondrial volume density, accumulation of lipofuscin, a product of lipid peroxidation, and dysregulation of enzymes whose time course is unknown. The effects of 7-9 days exposure to 4559 m (Margherita Hut, Monte Rosa, Italy) on the muscle proteins pattern were investigated, pre- and post-exposure, in ten young subjects, by 2-D DIGE and MS. Ten milligram biopsies were obtained from the mid part of the vastus lateralis muscle at sea level (control) and at altitude, after 7-9 days hypoxia. Differential analysis indicates that proteins involved in iron transport, tricarboxylic acid (TCA) cycle, oxidative phosphorylation, and oxidative stress responses were significantly (p

Original languageEnglish
Pages (from-to)4668-4679
Number of pages12
JournalProteomics
Volume8
Issue number22
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Oxidative stress
Muscle
Skeletal Muscle
Oxidative Stress
Modulation
Altitude Sickness
Mitochondrial Size
Lipids
Lipofuscin
Citric Acid Cycle
Muscle Proteins
Biopsy
Oxidative Phosphorylation
Level control
Quadriceps Muscle
Sea level
Heat-Shock Proteins
Oceans and Seas
Italy
Lipid Peroxidation

Keywords

  • 2-D DIGE
  • Hypoxia
  • Mass spectrometry
  • Muscle

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Proteins modulation in human skeletal muscle in the early phase of adaptation to hypobaric hypoxia. / Viganò, Agnese; Ripamonti, Marilena; De Palma, Sara; Capitanio, Daniele; Vasso, Michele; Wait, Robin; Lundby, Carsten; Cerretelli, Paolo; Gelfi, Cecilia.

In: Proteomics, Vol. 8, No. 22, 11.2008, p. 4668-4679.

Research output: Contribution to journalArticle

Viganò, A, Ripamonti, M, De Palma, S, Capitanio, D, Vasso, M, Wait, R, Lundby, C, Cerretelli, P & Gelfi, C 2008, 'Proteins modulation in human skeletal muscle in the early phase of adaptation to hypobaric hypoxia', Proteomics, vol. 8, no. 22, pp. 4668-4679. https://doi.org/10.1002/pmic.200800232
Viganò A, Ripamonti M, De Palma S, Capitanio D, Vasso M, Wait R et al. Proteins modulation in human skeletal muscle in the early phase of adaptation to hypobaric hypoxia. Proteomics. 2008 Nov;8(22):4668-4679. https://doi.org/10.1002/pmic.200800232
Viganò, Agnese ; Ripamonti, Marilena ; De Palma, Sara ; Capitanio, Daniele ; Vasso, Michele ; Wait, Robin ; Lundby, Carsten ; Cerretelli, Paolo ; Gelfi, Cecilia. / Proteins modulation in human skeletal muscle in the early phase of adaptation to hypobaric hypoxia. In: Proteomics. 2008 ; Vol. 8, No. 22. pp. 4668-4679.
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