Proteins of rat serum V: Adjuvant arthritis and its modulation by nonsteroidal anti-inflammatory drugs

Ivano Eberini, Davide Agnello, Ingrid Miller, Pia Villa, Maddalena Fratelli, Pietro Ghezzi, Manfred Gemeiner, Jason Chan, Ruedi Aebersold, Elisabetta Gianazza

Research output: Contribution to journalArticle

Abstract

The effect of adjuvant arthritis (AA) on the pattern of rat serum proteins includes the upregulation of haptoglobin, orosomucoid, α2- macroglobulin, serine protease inhibitor-3, thiostatin, α1-antitrypsin, C- reactive protein, and the downregulation of kallikrein-binding protein, α1- inhibitor III, apolipoprotein A-1, α2-HS-glycoprotein, albumin, apolipoprotein A-IV, transthyretin and transferrin. Minor changes (±20%) are observed for Gc-globulin, ceruloplasmin, and α1-macroglobulin. AA thus grossly resembles the acute inflammatory response elicited by the injection of turpentine, although the changes in the levels of negative acute-phase proteins (APP) are smaller in acute inflammation. Indomethacine and ibuprofen inhibit the effects of arthritis on the synthesis of rat serum proteins in different ways: The former is, on average, three times as effective as the latter. Each drug interferes differently with different proteins. In animals without AA, both nonsteroidal anti-inflammatory drugs (NSAID) mimick the inflammatory pattern to a certain extent, with more effect on the negative than on the positive APPs. Overall, the shifts in serum protein levels parallel changes in inflammatory parameters such as joint swelling and serum interleukin-6 (IL-6) activity. Protein quantitation after two-dimensional electrophoresis (2-DE) reveals some effects of the drugs per se which escape detection by other routine tests.

Original languageEnglish
Pages (from-to)2170-2179
Number of pages10
JournalElectrophoresis
Volume21
Issue number11
DOIs
Publication statusPublished - 2000

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Keywords

  • Acute phase reaction
  • Adjuvant arthritis
  • Ibuprofen
  • Indomethacine
  • Rat serum
  • Two-dimensional polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Eberini, I., Agnello, D., Miller, I., Villa, P., Fratelli, M., Ghezzi, P., Gemeiner, M., Chan, J., Aebersold, R., & Gianazza, E. (2000). Proteins of rat serum V: Adjuvant arthritis and its modulation by nonsteroidal anti-inflammatory drugs. Electrophoresis, 21(11), 2170-2179. https://doi.org/10.1002/1522-2683(20000601)21:11<2170::AID-ELPS2170>3.0.CO;2-1