Proteins phosphorylated on tyrosine as markers of human tumor cell lines

S. Giordano, M. F. Di Renzo, D. Cirillo, L. Naldini, L. Chiado'Piat, P. M. Comoglio

Research output: Contribution to journalArticle

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Abstract

Previous work has shown that proteins phosphorylated on tyrosine are selectively detectably by antibodies against phosphotyrosine (P-Tyr) in cells transformed by retroviral class-I oncogene-encoded kinases endowed with non-regulated activity (Di Renzo et al., 1986). In this work P-Tyr antibodies were used to investigate the existence of human tumors expressing abnormal levels of tyrosine phosphoproteins and tyrosine kinases. Among 18 cell lines examined, the antibodies identified a number of tumors with a detectable level of proteins phosphorylated on tyrosine. Among these were a major protein with an approximate M(r) of 150,000 in a gastric carcinoma; 2 proteins, with M(r) of 130,000 and 110,000 in a colon carcinoma; a major protein with M(r) of 170,000, tyrosine phosphorylated in both a urinary bladder and an epidermoid carcinoma; a 100,000 M(r) protein phosphorylated in lung and breast carcinomas. An 80,000 M(r) tyrosine phosphorylated protein was found in a fibrosarcoma and in a rhabdomyosarcoma. Among the hemopoietic malignancies screened, in 2 Philadelphia-positive chronic myelogenous leukemias P-Tyr antibodies recognized the chimeric bcr-abl 210,000 M(r) protein and its substrates. Two tyrosine phosphorylated proteins, one of M(r) 70,000 and one of M(r) 60,000, were detected in a Burkitt lymphoma line. These phosphoproteins were not found in samples harvested from normal gastro-intestinal or urinary bladder epithelium, nor in control fibroblasts and lymphocytes. Two of the above proteins have associated tyrosine kinase activity: the 170,000 M(r) protein of bladder carcinoma cells was found to be a constitutively phosphorylated EGF receptor.

Original languageEnglish
Pages (from-to)482-487
Number of pages6
JournalInternational Journal of Cancer
Volume39
Issue number4
DOIs
Publication statusPublished - 1987

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Tumor Cell Line
Tyrosine
Proteins
Phosphotyrosine
Urinary Bladder
Antibodies
Phosphoproteins
Carcinoma
Protein-Tyrosine Kinases
Neoplasms
Burkitt Lymphoma
Rhabdomyosarcoma
Fibrosarcoma
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Oncogenes
Epidermal Growth Factor Receptor
Squamous Cell Carcinoma
Stomach
Colon
Phosphotransferases

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Proteins phosphorylated on tyrosine as markers of human tumor cell lines. / Giordano, S.; Di Renzo, M. F.; Cirillo, D.; Naldini, L.; Chiado'Piat, L.; Comoglio, P. M.

In: International Journal of Cancer, Vol. 39, No. 4, 1987, p. 482-487.

Research output: Contribution to journalArticle

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