Proteolytic interconversion and N-terminal sequences of the Citrobacter diversus major β-lactamases

N. Franceschini, G. Amicosante, M. Perilli, M. Maccarrone, A. Oratore, J. Van Beeumen, J. M. Frere

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The N-terminal sequences of the two major β-lactamases produced by Citrobacter diversus differed only by the absence of the first residue in form II and the loss of five amino acid residues at the C-terminal end. Limited proteolysis of the homogeneous form I protein yielded a variety of enzymatically active products. In the major product obtained after the action of papain, the first three N-terminal residues of form I had been cleaved, whereas at the C-terminal end the treated enzyme lacked five residues. However, this cannot explain the different behaviours of form I, form II and papain digestion product upon chromatofocusing. Form I, which was sequenced up to position 56, exhibited a very high degree of similarity with a Klebsiella oxytoca β-lactamase. The determined sequence, which contained the active serine residue, demonstrated that the chromosome-encoded β-lactamase of Citrobacter diversus belong to class A.

Original languageEnglish
Pages (from-to)629-633
Number of pages5
JournalBiochemical Journal
Volume275
Issue number3
Publication statusPublished - 1991

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Citrobacter koseri
Papain
Klebsiella oxytoca
Proteolysis
Chromosomes
Serine
Digestion
Amino Acids
Enzymes
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Franceschini, N., Amicosante, G., Perilli, M., Maccarrone, M., Oratore, A., Van Beeumen, J., & Frere, J. M. (1991). Proteolytic interconversion and N-terminal sequences of the Citrobacter diversus major β-lactamases. Biochemical Journal, 275(3), 629-633.

Proteolytic interconversion and N-terminal sequences of the Citrobacter diversus major β-lactamases. / Franceschini, N.; Amicosante, G.; Perilli, M.; Maccarrone, M.; Oratore, A.; Van Beeumen, J.; Frere, J. M.

In: Biochemical Journal, Vol. 275, No. 3, 1991, p. 629-633.

Research output: Contribution to journalArticle

Franceschini, N, Amicosante, G, Perilli, M, Maccarrone, M, Oratore, A, Van Beeumen, J & Frere, JM 1991, 'Proteolytic interconversion and N-terminal sequences of the Citrobacter diversus major β-lactamases', Biochemical Journal, vol. 275, no. 3, pp. 629-633.
Franceschini N, Amicosante G, Perilli M, Maccarrone M, Oratore A, Van Beeumen J et al. Proteolytic interconversion and N-terminal sequences of the Citrobacter diversus major β-lactamases. Biochemical Journal. 1991;275(3):629-633.
Franceschini, N. ; Amicosante, G. ; Perilli, M. ; Maccarrone, M. ; Oratore, A. ; Van Beeumen, J. ; Frere, J. M. / Proteolytic interconversion and N-terminal sequences of the Citrobacter diversus major β-lactamases. In: Biochemical Journal. 1991 ; Vol. 275, No. 3. pp. 629-633.
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