Proteome adaptations in Ethe1-deficient mice indicate a role in lipid catabolism and cytoskeleton organization via post-translational protein modifications

Tatjana M. Hildebrandt, Ivano Di Meo, Massimo Zeviani, Carlo Viscomi, Hans Peter Braun

Research output: Contribution to journalArticle

Abstract

Hydrogen sulfide is a physiologically relevant signalling molecule. However, circulating levels of this highly biologically active substance have to be maintained within tightly controlled limits in order to avoid toxic side effects. In patients suffering from EE (ethylmalonic encephalopathy), a block in sulfide oxidation at the level of the SDO (sulfur dioxygenase) ETHE1 leads to severe dysfunctions in microcirculation and cellular energy metabolism. We used an Ethe1-deficient mouse model to investigate the effect of increased sulfide and persulfide concentrations on liver, kidney, muscle and brain proteomes. Major disturbances in post-translational protein modifications indicate that the mitochondrial sulfide oxidation pathway could have a crucial function during sulfide signalling most probably via the regulation of cysteine S-modifications. Our results confirm the involvement of sulfide in redox regulation and cytoskeleton dynamics. In addition, they suggest that sulfide signalling specifically regulates mitochondrial catabolism of FAs (fatty acids) and BCAAs (branched-chain amino acids). These findings are particularly relevant in the context of EE since they may explain major symptoms of the disease.

Original languageEnglish
Pages (from-to)575-584
Number of pages10
JournalBioscience Reports
Volume33
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

Sulfides
Proteome
Post Translational Protein Processing
Cytoskeleton
Lipids
Proteins
Microcirculation
Branched Chain Amino Acids
Oxidation
Hydrogen Sulfide
Poisons
Liver
Energy Metabolism
Oxidation-Reduction
Cysteine
Muscle
Brain
Fatty Acids
Kidney
Muscles

Keywords

  • Branched-chain amino acid oxidation
  • Ethylmalonic encephalopathy
  • Hydrogen sulfide
  • Mitochondria
  • Redox regulation
  • Sulfur dioxygenase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Proteome adaptations in Ethe1-deficient mice indicate a role in lipid catabolism and cytoskeleton organization via post-translational protein modifications. / Hildebrandt, Tatjana M.; Di Meo, Ivano; Zeviani, Massimo; Viscomi, Carlo; Braun, Hans Peter.

In: Bioscience Reports, Vol. 33, No. 4, 2013, p. 575-584.

Research output: Contribution to journalArticle

Hildebrandt, Tatjana M. ; Di Meo, Ivano ; Zeviani, Massimo ; Viscomi, Carlo ; Braun, Hans Peter. / Proteome adaptations in Ethe1-deficient mice indicate a role in lipid catabolism and cytoskeleton organization via post-translational protein modifications. In: Bioscience Reports. 2013 ; Vol. 33, No. 4. pp. 575-584.
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