Proteome of Bovine Mitochondria and Rod Outer Segment Disks: Commonalities and Differences

Maurizio Bruschi, Andrea Petretto, Federico Caicci, Martina Bartolucci, Daniela Calzia, Laura Santucci, Lucia Manni, Luca A. Ramenghi, Gianmarco Ghiggeri, Carlo E. Traverso, Giovanni Candiano, Isabella Panfoli

Research output: Contribution to journalArticlepeer-review

Abstract

The retinal rod outer segment (OS) is a stack of disks surrounded by the plasma membrane, housing proteins related to phototransduction, as well as mitochondrial proteins involved in oxidative phosphorylation (OxPhos). This prompted us to compare the proteome of bovine OS disks and mitochondria to assess the significant top gene signatures of each sample. The two proteomes, obtained by LTQ-Orbitrap Velos mass spectrometry, were compared by statistical analyses. In total, 4139 proteins were identified, 2045 of which overlapping in the two sets. Nonhierarchical Spearman's correlogram revealed that the groups were clearly discriminated. Partial least square discriminant plus support vector machine analysis identified the major discriminative proteins, implied in phototransduction and lipid metabolism, respectively. Gene Ontology analysis identified top gene signatures of the disk proteome, enriched in vesiculation, glycolysis, and OxPhos proteins. The tricarboxylic acid cycle and the electron transport proteins were similarly enriched in the two samples, but the latter was up regulated in disks. Data suggest that the mitochondrial OxPhos proteins may represent a true OS proteome component, outside the mitochondrion. This knowledge may help the scientific community in the further studies of retinal physiology and pathology.

Original languageEnglish
Pages (from-to)918-925
Number of pages8
JournalJournal of Proteome Research
Volume17
Issue number2
DOIs
Publication statusPublished - Feb 2 2018

Keywords

  • ATP synthase
  • disks
  • electron transfer chain
  • mass spectrometry
  • mitochondria
  • oxidative phosphorylation
  • rod outer segment
  • transmission electron microscopy

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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