Proteome of endothelial cell-derived procoagulant microparticles

Cristina Banfi, Maura Brioschi, Robin Wait, Shajna Begum, Elisabetta Gianazza, Angela Pirillo, Luciana Mussoni, Elena Tremoli

Research output: Contribution to journalArticle

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Abstract

Microparticles (MP) are small membrane vesicles that are released from cells upon activation or during apoptosis. Cellular MP in body fluids constitute a heterogeneous population, differing in cellular origin, numbers, size, antigenic composition and functional properties. MP support coagulation by exposure of tissue factor (TF), the initiator of coagulation in vivo. Moreover, MP may transfer bioactive molecules to other cells, thereby stimulating them to produce cytokines, cell-adhesion molecules, growth factors and TF, and modulate endothelial functions. However, a comprehensive characterization of the antigenic composition of MP has been poorly defined. This study describes the protein composition of endothelial cell (EC)-derived MP (EMP) using a proteomic approach. MS analysis indicated the presence of newly described protein such as metabolic enzymes, proteins involved in adhesion and fusion processes, members of protein folding event, cytoskeleton associated proteins and nucleosome. In conclusion, circulating EMP behave as an actual storage pool, able to disseminate blood-borne TF activity and other bioactive effectors, as confirmed by our experiments showing an increased procoagulant activity of EC exposed to EMP.

Original languageEnglish
Pages (from-to)4443-4455
Number of pages13
JournalProteomics
Volume5
Issue number17
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Cell-Derived Microparticles
Endothelial cells
Proteome
Thromboplastin
Endothelial Cells
Coagulation
Proteins
Chemical analysis
Protein folding
Nucleosomes
Body fluids
Protein Folding
Cell Adhesion Molecules
Body Fluids
Cytoskeleton
Proteomics
Intercellular Signaling Peptides and Proteins
Blood
Fusion reactions
Adhesion

Keywords

  • Endothelial cells
  • Microparticles

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Proteome of endothelial cell-derived procoagulant microparticles. / Banfi, Cristina; Brioschi, Maura; Wait, Robin; Begum, Shajna; Gianazza, Elisabetta; Pirillo, Angela; Mussoni, Luciana; Tremoli, Elena.

In: Proteomics, Vol. 5, No. 17, 11.2005, p. 4443-4455.

Research output: Contribution to journalArticle

Banfi, C, Brioschi, M, Wait, R, Begum, S, Gianazza, E, Pirillo, A, Mussoni, L & Tremoli, E 2005, 'Proteome of endothelial cell-derived procoagulant microparticles', Proteomics, vol. 5, no. 17, pp. 4443-4455. https://doi.org/10.1002/pmic.200402017
Banfi, Cristina ; Brioschi, Maura ; Wait, Robin ; Begum, Shajna ; Gianazza, Elisabetta ; Pirillo, Angela ; Mussoni, Luciana ; Tremoli, Elena. / Proteome of endothelial cell-derived procoagulant microparticles. In: Proteomics. 2005 ; Vol. 5, No. 17. pp. 4443-4455.
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