Proteome study of human cerebrospinal fluid following traumatic brain injury indicates fibrin(ogen) degradation products as trauma-associated markers

Antonio Conti, Yovan Sanchez-Ruiz, Angela Bachi, Luigi Beretta, Elisabetta Grandi, Massimiliano Beltramo, Massimo Alessio

Research output: Contribution to journalArticlepeer-review

Abstract

Traumatic brain injury (TBI), like other central nervous system pathologies, causes changes in the composition of cerebrospinal fluid (CSF). Consequently analysis of the CSF components is important to better understand the pathological processes involved in such diseases. The aim of this work was to identify specific markers of severe TBI. Proteomic analysis including two-dimensional gel electrophoresis combined with mass spectrometry analysis was used to compare the CSF protein profile of severe TBI patients and controls. Proteins (α 1 antitrypsin, haptoglobin 1 α1, α2, and β) belonging to the acute phase response showed an increased expression in severe TBI patients. Two other proteins, identified as proteolytic degradation products of the carboxyl-terminal portion of the fibrinogen β, were present only in TBI patients. The presence of these markers could correlate with a post-traumatic local increase in fibrinolysis as well as to an inflammatory event following CNS tissue injury.

Original languageEnglish
Pages (from-to)854-863
Number of pages10
JournalJournal of Neurotrauma
Volume21
Issue number7
DOIs
Publication statusPublished - Jul 2004

Keywords

  • Cerebrospinal fluid
  • Fibrinogen
  • Proteomics
  • Traumatic brain injury
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

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