Proteome study of human cerebrospinal fluid following traumatic brain injury indicates fibrin(ogen) degradation products as trauma-associated markers

Antonio Conti, Yovan Sanchez-Ruiz, Angela Bachi, Luigi Beretta, Elisabetta Grandi, Massimiliano Beltramo, Massimo Alessio

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Traumatic brain injury (TBI), like other central nervous system pathologies, causes changes in the composition of cerebrospinal fluid (CSF). Consequently analysis of the CSF components is important to better understand the pathological processes involved in such diseases. The aim of this work was to identify specific markers of severe TBI. Proteomic analysis including two-dimensional gel electrophoresis combined with mass spectrometry analysis was used to compare the CSF protein profile of severe TBI patients and controls. Proteins (α 1 antitrypsin, haptoglobin 1 α1, α2, and β) belonging to the acute phase response showed an increased expression in severe TBI patients. Two other proteins, identified as proteolytic degradation products of the carboxyl-terminal portion of the fibrinogen β, were present only in TBI patients. The presence of these markers could correlate with a post-traumatic local increase in fibrinolysis as well as to an inflammatory event following CNS tissue injury.

Original languageEnglish
Pages (from-to)854-863
Number of pages10
JournalJournal of Neurotrauma
Volume21
Issue number7
DOIs
Publication statusPublished - Jul 2004

Fingerprint

Fibrin Fibrinogen Degradation Products
Proteome
Cerebrospinal Fluid
Wounds and Injuries
Cerebrospinal Fluid Proteins
Acute-Phase Reaction
Haptoglobins
Electrophoresis, Gel, Two-Dimensional
Fibrinolysis
Pathologic Processes
Proteomics
Fibrinogen
Mass Spectrometry
Proteins
Central Nervous System
Traumatic Brain Injury
estropipate
Pathology

Keywords

  • Cerebrospinal fluid
  • Fibrinogen
  • Proteomics
  • Traumatic brain injury
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Proteome study of human cerebrospinal fluid following traumatic brain injury indicates fibrin(ogen) degradation products as trauma-associated markers. / Conti, Antonio; Sanchez-Ruiz, Yovan; Bachi, Angela; Beretta, Luigi; Grandi, Elisabetta; Beltramo, Massimiliano; Alessio, Massimo.

In: Journal of Neurotrauma, Vol. 21, No. 7, 07.2004, p. 854-863.

Research output: Contribution to journalArticle

Conti, Antonio ; Sanchez-Ruiz, Yovan ; Bachi, Angela ; Beretta, Luigi ; Grandi, Elisabetta ; Beltramo, Massimiliano ; Alessio, Massimo. / Proteome study of human cerebrospinal fluid following traumatic brain injury indicates fibrin(ogen) degradation products as trauma-associated markers. In: Journal of Neurotrauma. 2004 ; Vol. 21, No. 7. pp. 854-863.
@article{536f49163def4e62ace62c3363d24c59,
title = "Proteome study of human cerebrospinal fluid following traumatic brain injury indicates fibrin(ogen) degradation products as trauma-associated markers",
abstract = "Traumatic brain injury (TBI), like other central nervous system pathologies, causes changes in the composition of cerebrospinal fluid (CSF). Consequently analysis of the CSF components is important to better understand the pathological processes involved in such diseases. The aim of this work was to identify specific markers of severe TBI. Proteomic analysis including two-dimensional gel electrophoresis combined with mass spectrometry analysis was used to compare the CSF protein profile of severe TBI patients and controls. Proteins (α 1 antitrypsin, haptoglobin 1 α1, α2, and β) belonging to the acute phase response showed an increased expression in severe TBI patients. Two other proteins, identified as proteolytic degradation products of the carboxyl-terminal portion of the fibrinogen β, were present only in TBI patients. The presence of these markers could correlate with a post-traumatic local increase in fibrinolysis as well as to an inflammatory event following CNS tissue injury.",
keywords = "Cerebrospinal fluid, Fibrinogen, Proteomics, Traumatic brain injury, Two-dimensional gel electrophoresis",
author = "Antonio Conti and Yovan Sanchez-Ruiz and Angela Bachi and Luigi Beretta and Elisabetta Grandi and Massimiliano Beltramo and Massimo Alessio",
year = "2004",
month = "7",
doi = "10.1089/0897715041526212",
language = "English",
volume = "21",
pages = "854--863",
journal = "Journal of Neurotrauma",
issn = "0897-7151",
publisher = "Mary Ann Liebert Inc.",
number = "7",

}

TY - JOUR

T1 - Proteome study of human cerebrospinal fluid following traumatic brain injury indicates fibrin(ogen) degradation products as trauma-associated markers

AU - Conti, Antonio

AU - Sanchez-Ruiz, Yovan

AU - Bachi, Angela

AU - Beretta, Luigi

AU - Grandi, Elisabetta

AU - Beltramo, Massimiliano

AU - Alessio, Massimo

PY - 2004/7

Y1 - 2004/7

N2 - Traumatic brain injury (TBI), like other central nervous system pathologies, causes changes in the composition of cerebrospinal fluid (CSF). Consequently analysis of the CSF components is important to better understand the pathological processes involved in such diseases. The aim of this work was to identify specific markers of severe TBI. Proteomic analysis including two-dimensional gel electrophoresis combined with mass spectrometry analysis was used to compare the CSF protein profile of severe TBI patients and controls. Proteins (α 1 antitrypsin, haptoglobin 1 α1, α2, and β) belonging to the acute phase response showed an increased expression in severe TBI patients. Two other proteins, identified as proteolytic degradation products of the carboxyl-terminal portion of the fibrinogen β, were present only in TBI patients. The presence of these markers could correlate with a post-traumatic local increase in fibrinolysis as well as to an inflammatory event following CNS tissue injury.

AB - Traumatic brain injury (TBI), like other central nervous system pathologies, causes changes in the composition of cerebrospinal fluid (CSF). Consequently analysis of the CSF components is important to better understand the pathological processes involved in such diseases. The aim of this work was to identify specific markers of severe TBI. Proteomic analysis including two-dimensional gel electrophoresis combined with mass spectrometry analysis was used to compare the CSF protein profile of severe TBI patients and controls. Proteins (α 1 antitrypsin, haptoglobin 1 α1, α2, and β) belonging to the acute phase response showed an increased expression in severe TBI patients. Two other proteins, identified as proteolytic degradation products of the carboxyl-terminal portion of the fibrinogen β, were present only in TBI patients. The presence of these markers could correlate with a post-traumatic local increase in fibrinolysis as well as to an inflammatory event following CNS tissue injury.

KW - Cerebrospinal fluid

KW - Fibrinogen

KW - Proteomics

KW - Traumatic brain injury

KW - Two-dimensional gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=3242885511&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3242885511&partnerID=8YFLogxK

U2 - 10.1089/0897715041526212

DO - 10.1089/0897715041526212

M3 - Article

C2 - 15307898

AN - SCOPUS:3242885511

VL - 21

SP - 854

EP - 863

JO - Journal of Neurotrauma

JF - Journal of Neurotrauma

SN - 0897-7151

IS - 7

ER -