Proteomic analisys of protein extraction during hemofiltration with on-line endogenous reinfusion (HFR) using different polysulphone membranes

Monari Emanuela, Cuoghi Aurora, Bellei Elisa, Bergamini Stefania, Caiazzo Marialuisa, Aucella Filippo, Loschiavo Carmelo, Corazza Luca, Palladino Giuseppe, Sereni Luisa, Atti Mauro, Tomasi Aldo

Research output: Contribution to journalArticle

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Abstract

In end-stage renal disease patients, extracorporeal dialytic therapy is not able to prevent the accumulation of toxins related to the uremic syndrome, a severe complication that increases morbidity and mortality rate. In this paper, hemoFiltration with on-line Reinfusion (HFR) architecture is used to evaluate the effect of a more permeable membrane on the extraction of medium–high molecular weight molecules. The aim of this study was to compare two polysulphone membranes for convective chamber: polyphenylene High Flux (pHF) and polyphenylene Super High-Flux (pSHF). Fourteen patients were subjected to HFR with pHF and pSHF membranes and ultra filtrate (UF) samples were collected to evaluate molecular weight cut-off (MWCO) and to identify extracted proteins. Furthermore, image analysis software was used in order to evaluate change in protein extraction during the dialysis. The quantification of four proteins by immunoassay demonstrates a higher permeability of pSHF membrane. Two-dimensional electrophoresis (2-DE) gels showed, for both membranes, the greater number of protein spots at 235 min. Some of the identified proteins, involved in nephropathic disease complications, were compared to assess differences in extraction during dialytic treatment by PDQuest analysis. UF proteomic analysis demonstrated a different behavior for the two membranes; pHF membrane was more permeable at the beginning of HFR treatment (15 min), while pSHF membrane at the end of treatment (235 min). Proteomic analysis is a suitable approach to investigate the behavior of different membranes during dialysis. Results indicated that pSHF membrane offers the higher permeability, and showed higher efficiency in removal of middle molecules related to uremic syndrome.

Original languageEnglish
Pages (from-to)2691-2698
Number of pages8
JournalJournal of Materials Science: Materials in Medicine
Volume25
Issue number12
DOIs
Publication statusPublished - Nov 28 2014

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Hemofiltration
Polysulfones
Proteomics
Proteins
Membranes
Fluxes
Dialysis
Permeability
Dialysis membranes
Molecular weight
Molecular Weight
polysulfone P 1700
Molecules
polyphenylene sulfide
Electrophoresis
Therapeutics
Image analysis
Immunoassay
Chronic Kidney Failure
Gels

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering
  • Medicine(all)

Cite this

Proteomic analisys of protein extraction during hemofiltration with on-line endogenous reinfusion (HFR) using different polysulphone membranes. / Emanuela, Monari; Aurora, Cuoghi; Elisa, Bellei; Stefania, Bergamini; Marialuisa, Caiazzo; Filippo, Aucella; Carmelo, Loschiavo; Luca, Corazza; Giuseppe, Palladino; Luisa, Sereni; Mauro, Atti; Aldo, Tomasi.

In: Journal of Materials Science: Materials in Medicine, Vol. 25, No. 12, 28.11.2014, p. 2691-2698.

Research output: Contribution to journalArticle

Emanuela, M, Aurora, C, Elisa, B, Stefania, B, Marialuisa, C, Filippo, A, Carmelo, L, Luca, C, Giuseppe, P, Luisa, S, Mauro, A & Aldo, T 2014, 'Proteomic analisys of protein extraction during hemofiltration with on-line endogenous reinfusion (HFR) using different polysulphone membranes', Journal of Materials Science: Materials in Medicine, vol. 25, no. 12, pp. 2691-2698. https://doi.org/10.1007/s10856-014-5290-5
Emanuela, Monari ; Aurora, Cuoghi ; Elisa, Bellei ; Stefania, Bergamini ; Marialuisa, Caiazzo ; Filippo, Aucella ; Carmelo, Loschiavo ; Luca, Corazza ; Giuseppe, Palladino ; Luisa, Sereni ; Mauro, Atti ; Aldo, Tomasi. / Proteomic analisys of protein extraction during hemofiltration with on-line endogenous reinfusion (HFR) using different polysulphone membranes. In: Journal of Materials Science: Materials in Medicine. 2014 ; Vol. 25, No. 12. pp. 2691-2698.
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AU - Stefania, Bergamini

AU - Marialuisa, Caiazzo

AU - Filippo, Aucella

AU - Carmelo, Loschiavo

AU - Luca, Corazza

AU - Giuseppe, Palladino

AU - Luisa, Sereni

AU - Mauro, Atti

AU - Aldo, Tomasi

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