Proteomic analyses of the vitreous humour

Martina Angi, Helen Kalirai, Sarah E. Coupland, Bertil E. Damato, Francesco Semeraro, Mario R. Romano

Research output: Contribution to journalArticle

Abstract

The human vitreous humour (VH) is a transparent, highly hydrated gel, which occupies the posterior segment of the eye between the lens and the retina. Physiological and pathological conditions of the retina are reflected in the protein composition of the VH, which can be sampled as part of routine surgical procedures. Historically, many studies have investigated levels of individual proteins in VH from healthy and diseased eyes. In the last decade, proteomics analyses have been performed to characterise the proteome of the human VH and explore networks of functionally related proteins, providing insight into the aetiology of diabetic retinopathy and proliferative vitreoretinopathy. Recent proteomic studies on the VH from animal models of autoimmune uveitis have identified new signalling pathways associated to autoimmune triggers and intravitreal inflammation. This paper aims to guide biological scientists through the different proteomic techniques that have been used to analyse the VH and present future perspectives for the study of intravitreal inflammation using proteomic analyses.

Original languageEnglish
Article number148039
JournalMediators of Inflammation
Volume2012
DOIs
Publication statusPublished - 2012

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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    Angi, M., Kalirai, H., Coupland, S. E., Damato, B. E., Semeraro, F., & Romano, M. R. (2012). Proteomic analyses of the vitreous humour. Mediators of Inflammation, 2012, [148039]. https://doi.org/10.1155/2012/148039