Proteomic analyses reveal that Sky1 modulates apoptosis and mitophagy in Saccharomyces cerevisiae cells exposed to cisplatin

Silvia Rodríguez-Lombardero, M. Esther Rodríguez-Belmonte, M. Isabel González-Siso, Ángel Vizoso-Vázquez, Vanessa Valdiglesias, Blanca Laffón, M. Esperanza Cerdán

Research output: Contribution to journalArticle

Abstract

Sky1 is the only member of the SR (Serine-Arginine) protein kinase family in Saccharomyces cerevisiae. When yeast cells are treated with the anti-cancer drug cisplatin, Sky1 kinase activity is necessary to produce the cytotoxic effect. In this study, proteome changes in response to this drug and/or SKY1 deletion have been evaluated in order to understand the role of Sky1 in the response of yeast cells to cisplatin. Results reveal differential expression of proteins previously related to the oxidative stress response, DNA damage, apoptosis and mitophagy. With these precedents, the role of Sky1 in apoptosis, necrosis and mitophagy has been evaluated by flow-cytometry, fluorescence microscopy, biosensors and fluorescence techniques. After cisplatin treatment, an apoptotic-like process diminishes in the Δsky1 strain in comparison to the wild-type. The treatment does not affect mitophagy in the wild-type strain, while an increase is observed in the Δsky1 strain. The increased resistance to cisplatin observed in the Δsky1 strain may be attributable to a decrease of apoptosis and an increase of mitophagy.

Original languageEnglish
Pages (from-to)12573-12590
Number of pages18
JournalInternational Journal of Molecular Sciences
Volume15
Issue number7
DOIs
Publication statusPublished - Jul 15 2014

    Fingerprint

Keywords

  • Bmh1
  • Bmh2
  • Cancer
  • Chemoresistance
  • Yeast

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Medicine(all)

Cite this

Rodríguez-Lombardero, S., Rodríguez-Belmonte, M. E., González-Siso, M. I., Vizoso-Vázquez, Á., Valdiglesias, V., Laffón, B., & Cerdán, M. E. (2014). Proteomic analyses reveal that Sky1 modulates apoptosis and mitophagy in Saccharomyces cerevisiae cells exposed to cisplatin. International Journal of Molecular Sciences, 15(7), 12573-12590. https://doi.org/10.3390/ijms150712573