|Number of pages||14|
|Publication status||Published - 2017|
- Cerebrospinal fluid
- Liquid chromatography/electrospray tandem mass spectrometry
- Pediatric central nervous system tumors
- Protein-based biomarker
- Proteomic analysis
- collagen type 1
- core shell hydrogel nanoparticle
- glial cell line derived neurotrophic factor receptor
- glial cell line derived neurotrophic factor receptor alpha2
- inter alpha trypsin inhibitor
- inter alpha trypsin inhibitor heavy chain 4
- membrane protein
- neural proliferation and differentiation control protein 1
- procollagen C endopeptidase enhancer 1
- somatomedin binding protein 4
- unclassified drug
- case control study
- central nervous system cancer
- cerebrospinal fluid analysis
- controlled study
- disease association
- enzyme linked immunosorbent assay
- liquid chromatography-mass spectrometry
- major clinical study
- nonhodgkin lymphoma
- prospective study
- protein analysis
- protein microarray
- reverse phase protein array
- reversed phase liquid chromatography
- Western blotting
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Proteomic analysis of cerebrospinal fluid from children with central nervous system tumors identifies candidate proteins relating to tumor metastatic spread. / Spreafico, F.; Bongarzone, I.; Pizzamiglio, S. et al.In: Oncotarget, Vol. 8, No. 28, 2017, p. 46177-46190.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Proteomic analysis of cerebrospinal fluid from children with central nervous system tumors identifies candidate proteins relating to tumor metastatic spread
AU - Spreafico, F.
AU - Bongarzone, I.
AU - Pizzamiglio, S.
AU - Magni, R.
AU - Taverna, E.
AU - De Bortoli, M.
AU - Ciniselli, C.M.
AU - Barzanò, E.
AU - Biassoni, V.
AU - Luchini, A.
AU - Liotta, L.A.
AU - Zhou, W.
AU - Signore, M.
AU - Verderio, P.
AU - Massimino, M.
N1 - Cited By :2 Export Date: 5 April 2018 Correspondence Address: Pizzamiglio, S.; Unit of Medical Statistics, Biometry and Bioinformatics, Department of Applied Research and Technological Development, Fondazione IRCCS Istituto Nazionale dei TumoriItaly; email: email@example.com Chemicals/CAS: inter alpha trypsin inhibitor, 39346-44-6; somatomedin binding protein 4, 136653-35-5 References: Ichimura, K., Nishikawa, R., Matsutani, M., Molecular markers in pediatric Neuro Oncol (2012) Neuro-oncology, 14, pp. iv90-iv99; Northcott, P.A., Jones, D.T., Kool, M., Robinson, G.W., Gilbertson, R.J., Cho, Y.J., Pomeroy, S.L., Pfister, S.M., Medulloblastomics: the end of the beginning (2012) Nat Rev Cancer, 12, pp. 818-834; Massimino, M., Spreafico, F., Riva, D., Biassoni, V., Poggi, G., Solero, C., Gandola, L., Meazza, C., A lower-dose, lower-toxicity cisplatin-etoposide regimen for childhood progressive low-grade glioma (2010) J Neurooncol, 100, pp. 65-71; Dhall, G., Medulloblastoma (2009) J Child Neurol, 24, pp. 1418-1430; 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PY - 2017
Y1 - 2017
N2 - Central nervous system (CNS) tumors are the most common solid tumors in childhood. Since the sensitivity of combined cerebrospinal fluid (CSF) cytology and radiological neuroimaging in detecting meningeal metastases remains relatively low, we sought to characterize the CSF proteome of patients with CSF tumors to identify biomarkers predictive of metastatic spread. CSF samples from 27 children with brain tumors and 13 controls (extra-CNS non-Hodgkin lymphoma) were processed using core-shell hydrogel nanoparticles, and analyzed with reverse-phase liquid chromatography/electrospray tandem mass spectrometry (LC-MS/MS). Candidate proteins were identified with Fisher's exact test and/or a univariate logistic regression model. Reverse phase protein array (RPPA), Western blot (WB), and ELISA were used in the training set and in an independent set of CFS samples (60 cases, 14 controls) to validate our discovery findings. Among the 558 non-redundant proteins identified by LC-MS/MS, 147 were missing from the CSF database at http://www.biosino.org. Fourteen of the 26 final top-candidate proteins were chosen for validation with WB, RPPA and ELISA methods. Six proteins (type 1 collagen, insulin-like growth factor binding protein 4, procollagen C-endopeptidase enhancer 1, glial cell-line derived neurotrophic factor receptor α2, inter-alpha-trypsin inhibitor heavy chain 4, neural proliferation and differentiation control protein-1) revealed the ability to discriminate metastatic cases from controls. Combining a unique dataset of CSFs from pediatric CNS tumors with a novel enabling nanotechnology led us to identify CSF proteins potentially related to metastatic status. © Spreafico et al.
AB - Central nervous system (CNS) tumors are the most common solid tumors in childhood. Since the sensitivity of combined cerebrospinal fluid (CSF) cytology and radiological neuroimaging in detecting meningeal metastases remains relatively low, we sought to characterize the CSF proteome of patients with CSF tumors to identify biomarkers predictive of metastatic spread. CSF samples from 27 children with brain tumors and 13 controls (extra-CNS non-Hodgkin lymphoma) were processed using core-shell hydrogel nanoparticles, and analyzed with reverse-phase liquid chromatography/electrospray tandem mass spectrometry (LC-MS/MS). Candidate proteins were identified with Fisher's exact test and/or a univariate logistic regression model. Reverse phase protein array (RPPA), Western blot (WB), and ELISA were used in the training set and in an independent set of CFS samples (60 cases, 14 controls) to validate our discovery findings. Among the 558 non-redundant proteins identified by LC-MS/MS, 147 were missing from the CSF database at http://www.biosino.org. Fourteen of the 26 final top-candidate proteins were chosen for validation with WB, RPPA and ELISA methods. Six proteins (type 1 collagen, insulin-like growth factor binding protein 4, procollagen C-endopeptidase enhancer 1, glial cell-line derived neurotrophic factor receptor α2, inter-alpha-trypsin inhibitor heavy chain 4, neural proliferation and differentiation control protein-1) revealed the ability to discriminate metastatic cases from controls. Combining a unique dataset of CSFs from pediatric CNS tumors with a novel enabling nanotechnology led us to identify CSF proteins potentially related to metastatic status. © Spreafico et al.
KW - Cerebrospinal fluid
KW - Liquid chromatography/electrospray tandem mass spectrometry
KW - Pediatric central nervous system tumors
KW - Protein-based biomarker
KW - Proteomic analysis
KW - collagen type 1
KW - core shell hydrogel nanoparticle
KW - glial cell line derived neurotrophic factor receptor
KW - glial cell line derived neurotrophic factor receptor alpha2
KW - inter alpha trypsin inhibitor
KW - inter alpha trypsin inhibitor heavy chain 4
KW - membrane protein
KW - nanoparticle
KW - neural proliferation and differentiation control protein 1
KW - procollagen C endopeptidase enhancer 1
KW - proteome
KW - somatomedin binding protein 4
KW - unclassified drug
KW - adolescent
KW - adult
KW - Article
KW - case control study
KW - central nervous system cancer
KW - cerebrospinal fluid analysis
KW - child
KW - controlled study
KW - disease association
KW - enzyme linked immunosorbent assay
KW - female
KW - human
KW - liquid chromatography-mass spectrometry
KW - major clinical study
KW - male
KW - metastasis
KW - nanotechnology
KW - nonhodgkin lymphoma
KW - prospective study
KW - protein analysis
KW - protein microarray
KW - proteomics
KW - reverse phase protein array
KW - reversed phase liquid chromatography
KW - Western blotting
M3 - Article
VL - 8
SP - 46177
EP - 46190
JO - Oncotarget
JF - Oncotarget
SN - 1949-2553
IS - 28