Proteomic analysis of exhaled breath condensate from single patients with pulmonary emphysema associated to α1-antitrypsin deficiency

Marco Fumagalli, Lorenzo Dolcini, Alberto Sala, Jan Stolk, Laura Fregonese, Fabio Ferrari, Simona Viglio, Maurizio Luisetti, Paolo Iadarola

Research output: Contribution to journalArticlepeer-review

Abstract

The non-invasive character of exhaled breath (EBC) collection makes this fluid attractive for monitoring the respiratory tract by the measurement of various compounds. Because EBC is likely to reflect the composition of the airway-lining fluid, it can provide valuable information on possible disease states. Aim of our study was to apply proteomic technology to the study of EBC samples collected from single patients with pulmonary emphysema associated to α1-antitrypsin deficiency. The protein profiles from EBC of twenty patients and of twenty-five healthy individuals, used as controls, have been analyzed in parallel by a combination of 1-DE, 2-DE, μ-HPLC and MS. These sensitive techniques allowed to identify a number of cytokines and cytokeratins. Their level was found to be higher in patients than in controls.

Original languageEnglish
Pages (from-to)211-221
Number of pages11
JournalJournal of Proteomics
Volume71
Issue number2
DOIs
Publication statusPublished - Jul 21 2008

Keywords

  • α-antitrypsin deficiency
  • Cytokeratins
  • Cytokines
  • Exhaled breath condensate
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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