TY - JOUR
T1 - Proteomic analysis of sera from common variable immunodeficiency patients undergoing replacement intravenous immunoglobulin therapy
AU - Spadaro, Giuseppe
AU - D'Orio, Concetta
AU - Genovese, Arturo
AU - Galeotafiore, Antonella
AU - D'Ambrosio, Chiara
AU - Di Giovanni, Stefano
AU - Vitale, Monica
AU - Capasso, Mario
AU - Lamberti, Vincenzo
AU - Scaloni, Andrea
AU - Marone, Gianni
AU - Zambrano, Nicola
PY - 2011
Y1 - 2011
N2 - Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. By using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive, and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7, allowed to ascertain a potential role in the regulation of CLU gene expression by immunoglobulins.
AB - Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. By using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive, and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7, allowed to ascertain a potential role in the regulation of CLU gene expression by immunoglobulins.
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U2 - 10.1155/2011/706746
DO - 10.1155/2011/706746
M3 - Article
C2 - 21960740
AN - SCOPUS:81555212594
VL - 2011
JO - Journal of Biomedicine and Biotechnology
JF - Journal of Biomedicine and Biotechnology
SN - 1110-7243
M1 - 706746
ER -