Proteomic analysis reveals that apolipoprotein A1 levels are decreased in patients with Budd-Chiari syndrome

Simone Talens, Jildou Hoekstra, Steffen P G Dirkx, Sarwa Darwish Murad, Jonel Trebicka, Elwyn Elias, Massimo Primignani, Juan Carlos García-Pagán, Dominique C. Valla, Harry L A Janssen, Frank W G Leebeek, Dingeman C. Rijken

Research output: Contribution to journalArticle

Abstract

Background & Aims: Budd-Chiari syndrome (BCS) is a rare vascular liver disorder caused by thrombosis of the hepatic veins. In some patients, no known thrombophilic factor can be identified. This study aimed to identify novel factors that might play a role in thrombosis in BCS-patients by using a proteomic approach. Methods: The abundance of plasma clot-bound proteins was compared between nine BCS-patients and nine controls by using two-dimensional difference gel electrophoresis. The protein with the most significant decrease in patients was identified by mass spectrometry. Plasma levels of this protein were measured and the results were validated in a large cohort of BCS-patients. Results: A total of 26 protein spots significantly differed (p

Original languageEnglish
Pages (from-to)908-914
Number of pages7
JournalJournal of Hepatology
Volume54
Issue number5
DOIs
Publication statusPublished - May 2011

Keywords

  • 2D-DIGE
  • Apolipoprotein A1
  • Budd-Chiari syndrome
  • Proteomics
  • Venous thrombosis

ASJC Scopus subject areas

  • Hepatology

Fingerprint Dive into the research topics of 'Proteomic analysis reveals that apolipoprotein A1 levels are decreased in patients with Budd-Chiari syndrome'. Together they form a unique fingerprint.

  • Cite this

    Talens, S., Hoekstra, J., Dirkx, S. P. G., Murad, S. D., Trebicka, J., Elias, E., Primignani, M., García-Pagán, J. C., Valla, D. C., Janssen, H. L. A., Leebeek, F. W. G., & Rijken, D. C. (2011). Proteomic analysis reveals that apolipoprotein A1 levels are decreased in patients with Budd-Chiari syndrome. Journal of Hepatology, 54(5), 908-914. https://doi.org/10.1016/j.jhep.2010.08.026