Proteomic and genomic approaches to understanding the 'power plant' of cryptosporidium

Lorenza Putignani, Sanya J. Sanderson, Cristina Russo, Jessica Kissinger, Donato Menichella, Jonathan M. Wastling

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cryptosporidium spp. are important parasites of humans and animals for which current therapies are extremely limited. In order to target the biology of this unusual organism, a fuller understanding of its biochemistry, including the role of its subcellular organelles, is required. Genomics has enabled us to produce predictive biochemical maps of Cryptosporidium spp. and recently proteomic confirmation of many of these pathways has been achieved with the first full-scale proteomic analysis of Cryptosporidium parvum. These proteomic data, which cover over one-third of the entire predicted proteome, have now been made publicly available via CryptoDB (http://cryptodb.org/cryptodb/). Proteomic studies confirm that the parasite appears to retain a remnant organelle that fulfils some, but not all, of the roles of a fully functioning mitochondrion. Initial attempts to describe the sub-proteome of this organelle have met with limited success, and further work is required to improve the enrichment of the mitochondria-related organelle prior to proteomic analysis.

Original languageEnglish
Title of host publicationGiardia and Cryptosporidium: From Molecules to Diseases
PublisherCABI Publishing
Pages344-359
Number of pages16
ISBN (Print)9781845933913
Publication statusPublished - Feb 25 2009

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ASJC Scopus subject areas

  • veterinary(all)
  • Agricultural and Biological Sciences(all)

Cite this

Putignani, L., Sanderson, S. J., Russo, C., Kissinger, J., Menichella, D., & Wastling, J. M. (2009). Proteomic and genomic approaches to understanding the 'power plant' of cryptosporidium. In Giardia and Cryptosporidium: From Molecules to Diseases (pp. 344-359). CABI Publishing.