TY - JOUR
T1 - Proteomic Characterization of a New asymmetric Cellulose Triacetate Membrane for Hemodialysis
AU - Ronci, Maurizio
AU - Leporini, Lidia
AU - Felaco, Paolo
AU - Sirolli, Vittorio
AU - Pieroni, Luisa
AU - Greco, Viviana
AU - Aceto, Antonio
AU - Urbani, Andrea
AU - Bonomini, Mario
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Purpose: The artificial membrane inside the haemodialyzer is the main determinant of the quality and success of haemodialysis therapy. The performances of haemodialysis membranes are highly influenced by the interactions with plasma proteins, which in turn are related to the physical and chemical characteristics of the membrane material. The present cross-over study is aimed to analyse the haemodialysis performance of a newly developed asymmetric cellulose triacetate membrane (ATA) in comparison to the conventional parent symmetric polymer (CTA). Experimental design: In four chronic non diabetic haemodialysis patients, the protein constituents of the adsorbed material from the filters after the haemodialysis session, and the proteins recovered in the ultrafiltrate during the session, are identified using a bottom-up shotgun proteomics approach. Results: The ATA membrane shows a lower protein adsorption rate and a lower mass distribution pattern of the proteinaceous material. Conclusions and clinical relevance: By highlighting the differences between the two haemodialysis filters in terms of adsorbed proteins and flow through, it is demonstrated the higher biocompatibility of the novel ATA membrane, that fulfils the indications for the development of more performant membranes and may represent a step forward for the treatment of patients on chronic haemodialysis.
AB - Purpose: The artificial membrane inside the haemodialyzer is the main determinant of the quality and success of haemodialysis therapy. The performances of haemodialysis membranes are highly influenced by the interactions with plasma proteins, which in turn are related to the physical and chemical characteristics of the membrane material. The present cross-over study is aimed to analyse the haemodialysis performance of a newly developed asymmetric cellulose triacetate membrane (ATA) in comparison to the conventional parent symmetric polymer (CTA). Experimental design: In four chronic non diabetic haemodialysis patients, the protein constituents of the adsorbed material from the filters after the haemodialysis session, and the proteins recovered in the ultrafiltrate during the session, are identified using a bottom-up shotgun proteomics approach. Results: The ATA membrane shows a lower protein adsorption rate and a lower mass distribution pattern of the proteinaceous material. Conclusions and clinical relevance: By highlighting the differences between the two haemodialysis filters in terms of adsorbed proteins and flow through, it is demonstrated the higher biocompatibility of the novel ATA membrane, that fulfils the indications for the development of more performant membranes and may represent a step forward for the treatment of patients on chronic haemodialysis.
KW - adsorption
KW - cellulose triacetate membrane
KW - hemodialysis
KW - liquid chromatography tandem mass spectrometry
KW - protein identification
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U2 - 10.1002/prca.201700140
DO - 10.1002/prca.201700140
M3 - Article
C2 - 29808585
AN - SCOPUS:85053790245
VL - 12
JO - Proteomics - Clinical Applications
JF - Proteomics - Clinical Applications
SN - 1862-8346
IS - 6
M1 - 1700140
ER -