Proteomic identification of plasma biomarkers in children and adolescents with recurrent hodgkin lymphoma

Ombretta Repetto, Lara Mussolin, Caterina Elia, Lia Martina, Maurizio Bianchi, Salvatore Buffardi, Alessandra Sala, Roberta Burnelli, Maurizio Mascarin, Valli De Re

Research output: Contribution to journalArticle

Abstract

The treatment of paediatric Hodgkin lymphoma (HL) has steadily improved over the years, so that 10-years survival exceed 80%. The purpose of this study was to identify prognostic markers for relapsed HL that might contribute to optimize therapeutic approaches. To this aim we retrospectively analysed differential protein expression profiles obtained from plasma of children/adolescents with HL (age ranging from 10 to 18 years) collected at diagnosis. We examined the protein profiles of 15 HL relapsed (R) patients compared with 14 HL not relapsed (NR) patients treated with the same LH-2004 protocol. Two dimensional difference in gel electrophoresis (2D-DIGE) revealed significant differences (fold change > 1.5; Student's T-test p<0.01) between R and NR patients in 10 proteins: α-1-antitrypsin chain a, apolipoprotein A-IV precursor; inter-α-trypsin inhibitor heavy chain; antithrombin-III; vitronectin; fibrinogen α, β and γ chains, complement C3, and ceruloplasmin. An up-regulation of fibrinogen α (spots 78, 196, 230, 234, 239) and β (spots 98, 291, 296, 300) chains together with a lower level of α-1-antitrypsin (spots 255, 264, 266, 272, 273) were found in R patients, and this difference was validated by immunoblotting. The functional role(s) of these proteins in the coagulation and inflammation associated with paediatric/adolescent HL progression and relapse deserves further investigations.

Original languageEnglish
Pages (from-to)4650-4658
Number of pages9
JournalJournal of Cancer
Volume9
Issue number24
DOIs
Publication statusPublished - Jan 1 2018

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Keywords

  • Biomarkers
  • DIGE
  • Paediatric Hodgkin lymphoma
  • Plasma proteins
  • Relapse

ASJC Scopus subject areas

  • Oncology

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