Proteomic investigation in A549 lung cell line stably infected by HPV16E6/E7 oncogenes

Marco Ciotti, Valeria Marzano, Laura Giuliani, Marzia Nuccetelli, Simona D'Aguanno, Barbara Azzimonti, Sergio Bernardini, Carlo Federico Perno, Andrea Urbani, Cartesio Favalli, Giorgio Federici

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Data have accumulated implicating the involvement of oncogenic human papillomaviruses (HPVs) in bronchial carcinogenesis. We recently described the presence of oncogenic HPV transcripts in non-small cell lung cancers. Objective: To investigate the role of oncogenic HPVs in lung carcinogenesis. Material and Methods: The lung cell line A549 stably infected with HPV16E6, HPV16E7 and HPVE6/E7 constructs was used to investigate the protein profile changes associated with the expression of these oncogenes. Replicated two-dimensional gel electrophoresis gels from uninfected and stably HPV16E6-, E7-, and E6/E7-infected A549 cells were compared for changes in protein profile. Protein identification was achieved by peptide mass fingerprinting by MALDI-TOF-MS and nLC-ESI-Q-TOF-MS/MS peptide ladder sequencing. Results: We identified 17 different polypeptides whose average normalized spot intensity was statistically significant (p <0.05) and differed by 2-fold. Relationships between differentially expressed proteins and the HPV-induced infection mechanism have been clustered by knowledge-base database functional association network analysis. Conclusion: The impact of Hsp27, annexin III, annexin IV, Gp96 and TPT1 on the cellular response mechanism to HPV infection is presented and discussed.

Original languageEnglish
Pages (from-to)427-439
Number of pages13
JournalRespiration
Volume77
Issue number4
DOIs
Publication statusPublished - May 2009

Fingerprint

Oncogenes
Proteomics
Lung
Papillomavirus Infections
Annexin A3
Annexin A4
Carcinogenesis
Proteins
Peptides
Peptide Mapping
Knowledge Bases
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
Non-Small Cell Lung Carcinoma
Gels
A549 Cells
Databases

Keywords

  • Human
  • Lung cancer
  • Mass spectrometry
  • Molecular networks
  • Oncogene
  • Papillomaviruses
  • Proteomics

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Ciotti, M., Marzano, V., Giuliani, L., Nuccetelli, M., D'Aguanno, S., Azzimonti, B., ... Federici, G. (2009). Proteomic investigation in A549 lung cell line stably infected by HPV16E6/E7 oncogenes. Respiration, 77(4), 427-439. https://doi.org/10.1159/000176209

Proteomic investigation in A549 lung cell line stably infected by HPV16E6/E7 oncogenes. / Ciotti, Marco; Marzano, Valeria; Giuliani, Laura; Nuccetelli, Marzia; D'Aguanno, Simona; Azzimonti, Barbara; Bernardini, Sergio; Perno, Carlo Federico; Urbani, Andrea; Favalli, Cartesio; Federici, Giorgio.

In: Respiration, Vol. 77, No. 4, 05.2009, p. 427-439.

Research output: Contribution to journalArticle

Ciotti, M, Marzano, V, Giuliani, L, Nuccetelli, M, D'Aguanno, S, Azzimonti, B, Bernardini, S, Perno, CF, Urbani, A, Favalli, C & Federici, G 2009, 'Proteomic investigation in A549 lung cell line stably infected by HPV16E6/E7 oncogenes', Respiration, vol. 77, no. 4, pp. 427-439. https://doi.org/10.1159/000176209
Ciotti, Marco ; Marzano, Valeria ; Giuliani, Laura ; Nuccetelli, Marzia ; D'Aguanno, Simona ; Azzimonti, Barbara ; Bernardini, Sergio ; Perno, Carlo Federico ; Urbani, Andrea ; Favalli, Cartesio ; Federici, Giorgio. / Proteomic investigation in A549 lung cell line stably infected by HPV16E6/E7 oncogenes. In: Respiration. 2009 ; Vol. 77, No. 4. pp. 427-439.
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