Proteomic investigation in A549 lung cell line stably infected by HPV16E6/E7 oncogenes

Marco Ciotti, Valeria Marzano, Laura Giuliani, Marzia Nuccetelli, Simona D'Aguanno, Barbara Azzimonti, Sergio Bernardini, Carlo Federico Perno, Andrea Urbani, Cartesio Favalli, Giorgio Federici

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Data have accumulated implicating the involvement of oncogenic human papillomaviruses (HPVs) in bronchial carcinogenesis. We recently described the presence of oncogenic HPV transcripts in non-small cell lung cancers. Objective: To investigate the role of oncogenic HPVs in lung carcinogenesis. Material and Methods: The lung cell line A549 stably infected with HPV16E6, HPV16E7 and HPVE6/E7 constructs was used to investigate the protein profile changes associated with the expression of these oncogenes. Replicated two-dimensional gel electrophoresis gels from uninfected and stably HPV16E6-, E7-, and E6/E7-infected A549 cells were compared for changes in protein profile. Protein identification was achieved by peptide mass fingerprinting by MALDI-TOF-MS and nLC-ESI-Q-TOF-MS/MS peptide ladder sequencing. Results: We identified 17 different polypeptides whose average normalized spot intensity was statistically significant (p <0.05) and differed by 2-fold. Relationships between differentially expressed proteins and the HPV-induced infection mechanism have been clustered by knowledge-base database functional association network analysis. Conclusion: The impact of Hsp27, annexin III, annexin IV, Gp96 and TPT1 on the cellular response mechanism to HPV infection is presented and discussed.

Original languageEnglish
Pages (from-to)427-439
Number of pages13
JournalRespiration
Volume77
Issue number4
DOIs
Publication statusPublished - May 2009

Keywords

  • Human
  • Lung cancer
  • Mass spectrometry
  • Molecular networks
  • Oncogene
  • Papillomaviruses
  • Proteomics

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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