Proteomic profile of the nucellus of castor bean (Ricinus communis L.) seeds during development

Fábio C S Nogueira, Giuseppe Palmisano, Emanoella L. Soares, Mohibullah Shah, Arlete A. Soares, Peter Roepstorff, Francisco A P Campos, Gilberto B. Domont

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In this study, we performed a proteomic analysis of nucellus from two developmental stages of Ricinus communis seeds by a GeLC-MS/MS approach, using of a high resolution orbitrap mass spectrometer, which resulted in the identification of a total of 766 proteins that were grouped into 553 protein groups. The distribution of the identified proteins in stages III and IV into different Gene Ontology categories was similar, with a remarkable abundance of proteins associated with the protein synthesis machinery of cells, as well as several classes of proteins involved in protein degradation, particularly of peptidases associated with programmed cell death. Consistent with the role of the nucellus in mediating nutrient transfer from maternal tissues to the endosperm and embryo, a significant proportion of the identified proteins are related to amino acid metabolism, but none of the identified proteins are known to have a role as storage proteins. Moreover for the first time, ricin isoforms were identified in tissues other than seed endosperm. Results are discussed in the context of the spatial and temporal distribution of the identified proteins within the nucellar cell layers.

Original languageEnglish
Pages (from-to)1933-1939
Number of pages7
JournalJournal of Proteomics
Volume75
Issue number6
DOIs
Publication statusPublished - Mar 16 2012

Fingerprint

Castor Bean
Ricinus
Proteomics
Seed
Seeds
Proteins
Endosperm
Ricin
Tissue
Gene Ontology
Mass spectrometers
Cell death
Proteolysis
Metabolism
Nutrients
Protein Isoforms
Machinery
Ontology
Peptide Hydrolases
Cell Death

Keywords

  • Castor bean
  • Nucellus
  • Oil seed
  • Plant proteomics
  • Programmed cell death
  • Ricinus communis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Nogueira, F. C. S., Palmisano, G., Soares, E. L., Shah, M., Soares, A. A., Roepstorff, P., ... Domont, G. B. (2012). Proteomic profile of the nucellus of castor bean (Ricinus communis L.) seeds during development. Journal of Proteomics, 75(6), 1933-1939. https://doi.org/10.1016/j.jprot.2012.01.002

Proteomic profile of the nucellus of castor bean (Ricinus communis L.) seeds during development. / Nogueira, Fábio C S; Palmisano, Giuseppe; Soares, Emanoella L.; Shah, Mohibullah; Soares, Arlete A.; Roepstorff, Peter; Campos, Francisco A P; Domont, Gilberto B.

In: Journal of Proteomics, Vol. 75, No. 6, 16.03.2012, p. 1933-1939.

Research output: Contribution to journalArticle

Nogueira, FCS, Palmisano, G, Soares, EL, Shah, M, Soares, AA, Roepstorff, P, Campos, FAP & Domont, GB 2012, 'Proteomic profile of the nucellus of castor bean (Ricinus communis L.) seeds during development', Journal of Proteomics, vol. 75, no. 6, pp. 1933-1939. https://doi.org/10.1016/j.jprot.2012.01.002
Nogueira FCS, Palmisano G, Soares EL, Shah M, Soares AA, Roepstorff P et al. Proteomic profile of the nucellus of castor bean (Ricinus communis L.) seeds during development. Journal of Proteomics. 2012 Mar 16;75(6):1933-1939. https://doi.org/10.1016/j.jprot.2012.01.002
Nogueira, Fábio C S ; Palmisano, Giuseppe ; Soares, Emanoella L. ; Shah, Mohibullah ; Soares, Arlete A. ; Roepstorff, Peter ; Campos, Francisco A P ; Domont, Gilberto B. / Proteomic profile of the nucellus of castor bean (Ricinus communis L.) seeds during development. In: Journal of Proteomics. 2012 ; Vol. 75, No. 6. pp. 1933-1939.
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