Proteomic profiling of PrP27-30-enriched preparations extracted from the brain of hamsters with experimental scrapie

Alessandra Giorgi, Laura Di Francesco, Serena Principe, Giuseppina Mignogna, Lau Sennels, Carmine Mancone, Tonino Alonzi, Marco Sbriccoli, Angela De Pascalis, Juri Rappsilber, Franco Cardone, Maurizio Pocchiari, Bruno Maras, M. Eugenia Schinina

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Transmissible spongiform encephalopathies (TSEs) are neurodegenerative disorders characterized by the accumulation in the CNS of a pathological conformer (PrPTSE) of the host-encoded cellular prion protein (PrPC). PrPTSE has a central role in the pathogenesis of the disease but other factors are likely involved in the pathological process. In this work we employed a multi-step proteomic approach for the identification of proteins that co-purify with the protease-resistant core of PrPTSE (PrP27-30) extracted from brains of hamsters with experimental scrapie. We identified ferritin, calcium/calmodulin-dependent protein kinase α type II, apolipoprotein E, and tubulin as the major components associated with PrP27-30 but also trace amounts of actin, cofilin, Hsp90α, the γ subunit of the T-complex protein 1, glyceraldehyde 3-phosphate dehydrogenase, histones, and keratins. Whereas some of these proteins (tubulin and ferritin) are known to bind PrP, other proteins (calcium/calmodulin-dependent protein kinase α type II, Hsp90α) may associate with PrPTSE fibrils during disease. Apolipoprotein E and actin have been previously observed in association with PrPTSE, whereas cofilin and actin were shown to form abnormal rods in the brain of patients with Alzheimer disease. The roles of these proteins in the development of brain lesions are still unclear and further work is needed to explain their involvement in the pathogenesis of TSEs.

Original languageEnglish
Pages (from-to)3802-3814
Number of pages13
JournalProteomics
Volume9
Issue number15
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Scrapie
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Cricetinae
Proteomics
Brain
Actin Depolymerizing Factors
Actins
Calcium-Calmodulin-Dependent Protein Kinases
Prion Diseases
Apolipoproteins E
Tubulin
Ferritins
PrPC Proteins
Proteins
Glyceraldehyde-3-Phosphate Dehydrogenases
Pathologic Processes
Keratins
Neurodegenerative Diseases
Histones
Alzheimer Disease

Keywords

  • FT-ICR
  • MALDI-TOF/TOF
  • Prion protein
  • PrP27-30
  • Transmissible spongiform encephalopathies

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Giorgi, A., Di Francesco, L., Principe, S., Mignogna, G., Sennels, L., Mancone, C., ... Schinina, M. E. (2009). Proteomic profiling of PrP27-30-enriched preparations extracted from the brain of hamsters with experimental scrapie. Proteomics, 9(15), 3802-3814. https://doi.org/10.1002/pmic.200900085

Proteomic profiling of PrP27-30-enriched preparations extracted from the brain of hamsters with experimental scrapie. / Giorgi, Alessandra; Di Francesco, Laura; Principe, Serena; Mignogna, Giuseppina; Sennels, Lau; Mancone, Carmine; Alonzi, Tonino; Sbriccoli, Marco; De Pascalis, Angela; Rappsilber, Juri; Cardone, Franco; Pocchiari, Maurizio; Maras, Bruno; Schinina, M. Eugenia.

In: Proteomics, Vol. 9, No. 15, 08.2009, p. 3802-3814.

Research output: Contribution to journalArticle

Giorgi, A, Di Francesco, L, Principe, S, Mignogna, G, Sennels, L, Mancone, C, Alonzi, T, Sbriccoli, M, De Pascalis, A, Rappsilber, J, Cardone, F, Pocchiari, M, Maras, B & Schinina, ME 2009, 'Proteomic profiling of PrP27-30-enriched preparations extracted from the brain of hamsters with experimental scrapie', Proteomics, vol. 9, no. 15, pp. 3802-3814. https://doi.org/10.1002/pmic.200900085
Giorgi, Alessandra ; Di Francesco, Laura ; Principe, Serena ; Mignogna, Giuseppina ; Sennels, Lau ; Mancone, Carmine ; Alonzi, Tonino ; Sbriccoli, Marco ; De Pascalis, Angela ; Rappsilber, Juri ; Cardone, Franco ; Pocchiari, Maurizio ; Maras, Bruno ; Schinina, M. Eugenia. / Proteomic profiling of PrP27-30-enriched preparations extracted from the brain of hamsters with experimental scrapie. In: Proteomics. 2009 ; Vol. 9, No. 15. pp. 3802-3814.
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AU - Alonzi, Tonino

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AU - De Pascalis, Angela

AU - Rappsilber, Juri

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