Proteomic snapshot of the EGF-induced ubiquitin network

Elisabetta Argenzio, Tanja Bange, Barbara Oldrini, Fabrizio Bianchi, Raghunath Peesari, Sara Mari, Pier Paolo Di Fiore, Matthias Mann, Simona Polo

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The activity, localization and fate of many cellular proteins are regulated through ubiquitination, a process whereby one or more ubiquitin (Ub) monomers or chains are covalently attached to target proteins. While Ub-conjugated and Ub-associated proteomes have been described, we lack a high-resolution picture of the dynamics of ubiquitination in response to signaling. In this study, we describe the epidermal growth factor (EGF)-regulated Ubiproteome, as obtained by two complementary purification strategies coupled to quantitative proteomics. Our results unveil the complex impact of growth factor signaling on Ub-based intracellular networks to levels that extend well beyond what might have been expected. In addition to endocytic proteins, the EGF-regulated Ubiproteome includes a large number of signaling proteins, ubiquitinating and deubiquitinating enzymes, transporters and proteins involved in translation and transcription. The Ub-based signaling network appears to intersect both housekeeping and regulatory circuitries of cellular physiology. Finally, as proof of principle of the biological relevance of the EGF-Ubiproteome, we demonstrated that EphA2 is a novel, downstream ubiquitinated target of epidermal growth factor receptor (EGFR), critically involved in EGFR biological responses.

Original languageEnglish
Article number462
JournalMolecular Systems Biology
Volume7
DOIs
Publication statusPublished - 2011

Fingerprint

Growth Factors
Proteomics
epidermal growth factor
Snapshot
ubiquitin
Ubiquitin
Epidermal Growth Factor
proteomics
Proteins
Protein
Ubiquitination
Epidermal Growth Factor Receptor
proteins
Receptor
Housekeeping
Physiology
Proteome
Transcription
proteome
Target

Keywords

  • EGF
  • network
  • proteomics
  • signaling
  • ubiquitin

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Computational Theory and Mathematics
  • Information Systems
  • Applied Mathematics

Cite this

Proteomic snapshot of the EGF-induced ubiquitin network. / Argenzio, Elisabetta; Bange, Tanja; Oldrini, Barbara; Bianchi, Fabrizio; Peesari, Raghunath; Mari, Sara; Di Fiore, Pier Paolo; Mann, Matthias; Polo, Simona.

In: Molecular Systems Biology, Vol. 7, 462, 2011.

Research output: Contribution to journalArticle

Argenzio, Elisabetta ; Bange, Tanja ; Oldrini, Barbara ; Bianchi, Fabrizio ; Peesari, Raghunath ; Mari, Sara ; Di Fiore, Pier Paolo ; Mann, Matthias ; Polo, Simona. / Proteomic snapshot of the EGF-induced ubiquitin network. In: Molecular Systems Biology. 2011 ; Vol. 7.
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