Proteomics-based evidence for a pro-oncogenic role of ESRP1 in human colorectal cancer cells

Ugo Ala, Marta Manco, Giorgia Mandili, Emanuela Tolosano, Francesco Novelli, Paolo Provero, Fiorella Altruda, Sharmila Fagoonee

Research output: Contribution to journalArticlepeer-review

Abstract

The RNA-binding protein, Epithelial Splicing Regulatory Protein 1 (ESRP1) can promote or suppress tumorigenesis depending on the cell type and disease context. In colorectal cancer, we have previously shown that aberrantly high ESRP1 expression can drive tumor progression. In order to unveil the mechanisms by which ESRP1 can modulate cancer traits, we searched for proteins affected by modulation of Esrp1 in two human colorectal cancer cell lines, HCA24 and COLO320DM, by proteomics analysis. Proteins hosted by endogenous ESRP1 ribonucleoprotein complex in HCA24 cells were also analyzed following RNA-immunoprecipitation. Proteomics data were complemented with bioinformatics approach to exploit publicly available data on protein-protein interaction (PPI). Gene Ontology was analysed to identify a common molecular signature possibly explaining the pro-tumorigenic role of ESRP1. Interestingly, proteins identified herein support a role for ESRP1 in response to external stimulus, regulation of cell cycle and hypoxia. Our data provide further insights into factors affected by and entwined with ESRP1 in colorectal cancer.

Original languageEnglish
Article number575
JournalInternational Journal of Molecular Sciences
Volume21
Issue number2
DOIs
Publication statusPublished - Jan 2 2020

Keywords

  • Bioinformatics
  • Colorectal cancer
  • ESRP1
  • Proteomics

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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