Proteomics characterization of protein adsorption onto hemodialysis membranes

Mario Bonomini, Barbara Pavone, Vittorio Sirolli, Francesca Del Buono, Moreno Di Cesare, Piero Del Boccio, Luigi Amoroso, Carmine Di Ilio, Paolo Sacchetta, Giorgio Federici, Andrea Urbani

Research output: Contribution to journalArticlepeer-review

Abstract

Protein-adsorptive properties are a key feature of membranes used for hemodialysis treatment. Protein adsorption is vital to the biocompatibility of a membrane material and influences membrane's performance. The object of the present study is to investigate membrane biocompatibility by correlating the adsorbed proteome repertoire with structural feature of the membrane surfaces. Minidialyzers of identical structural characteristics composed of either cellulose diacetate or ethylenevinyl alcohol materials were employed to develop an ex vivo apparatus to investigate protein adsorption. Adsorbed proteins were eluted by a strong chaotropic buffer condition and investigated by 2-DE coupled to both MALDI-TOF mass spectrometry (MS) mass fingerprinting and fragmentation analysis on a nanoLC-MS/MS hybrid instrument. Membrane surface characterization included evaluation of roughness (atomic force microscopy), elemental chemical composition (X-ray-photoelectron-spectroscopy), and hydrophilicity (pulsed nuclear magnetic resonance). The present study identifies a number of different proteins as common or characteristic of filter material interaction, showing that proteomic techniques are a promising approach for the investigation of proteins surface-adsorbed onto hemodialysis membrane. Proteomic analysis enables the characterization of protein layers of unknown composition.

Original languageEnglish
Pages (from-to)2666-2674
Number of pages9
JournalJournal of Proteome Research
Volume5
Issue number10
DOIs
Publication statusPublished - Oct 2006

Keywords

  • Hemodialysis
  • Mass spectrometry
  • Membrane material
  • Minidialyzer
  • Protein adsorption
  • Proteomics

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

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