Proteomics for the identification of specifically oxidized proteins in brain: Technology and application to the study of neurodegenerative disorders

D. A. Butterfield, A. Castegna

Research output: Contribution to journalReview article

Abstract

Proteomics offers the opportunity elucidate the complex protein interactions of cellular systems by studying the products of genes, i.e., proteins, and their structure, function and localization. The purpose of proteomics is to explain the information contained in the genome sequences in order to provide clues on cellular events, especially related to disease. Our proteomic approach has made possible the identification of specifically oxidized proteins in Alzheimers disease (AD) brain, providing for the first time evidence on how oxidative stress plays a crucial role in AD-related neurodegeneration. This represents an example of the use of proteomics to solve biological problems related to disease. The field, which is still in its infancy, represents a very promising way to elucidate mechanism of disease at a protein level. However, the techniques that support its development present several limitations and require introduction of new tools and innovation in order to achieve a fast, reliable and sensitive method to understand normal biological processes and their regulation as well as these cellular properties in disease.

Original languageEnglish
Pages (from-to)419-425
Number of pages7
JournalAmino Acids
Volume25
Issue number3-4
DOIs
Publication statusPublished - Dec 2003

Fingerprint

Neurodegenerative Diseases
Proteomics
Brain
Technology
Alzheimer Disease
Proteins
Biological Phenomena
Genes
Oxidative Stress
Oxidative stress
Genome
Innovation

Keywords

  • Alzheimer's disease
  • Neurodegeneration
  • Oxidized proteins
  • Proteomics

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry
  • Endocrinology

Cite this

Proteomics for the identification of specifically oxidized proteins in brain : Technology and application to the study of neurodegenerative disorders. / Butterfield, D. A.; Castegna, A.

In: Amino Acids, Vol. 25, No. 3-4, 12.2003, p. 419-425.

Research output: Contribution to journalReview article

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