TY - JOUR
T1 - Proteomics investigation of human platelets in healthy donors and cystic fibrosis patients by shotgun nUPLC-MSE and 2DE
T2 - A comparative study
AU - Pieroni, Luisa
AU - Finamore, Francesco
AU - Ronci, Maurizio
AU - Mattoscio, Domenico
AU - Marzano, Valeria
AU - Mortera, Stefano Levi
AU - Quattrucci, Serena
AU - Federici, Giorgio
AU - Romano, Mario
AU - Urbani, Andrea
PY - 2011/3/1
Y1 - 2011/3/1
N2 - Platelets are of pathophysiological relevance in haemostasis, wound repair, inflammation and cardiovascular disease. We have shown that human platelets express a biologically active Cystic Fibrosis Transmembrane Conductance Regulator, which is dysfunctional in Cystic Fibrosis (CF) patients, and regulate platelet responses related to inflammation and its resolution. In order to further elucidate platelet involvement in CF inflammation, we pursued a comparative proteomic analysis of cells from healthy donors and CF patients, in association with a non-supervised comparative analysis of the Gene Ontology. Our results, showing changes in the integrin signalling in CF, support a pro-inflammatory profile of CF platelets.
AB - Platelets are of pathophysiological relevance in haemostasis, wound repair, inflammation and cardiovascular disease. We have shown that human platelets express a biologically active Cystic Fibrosis Transmembrane Conductance Regulator, which is dysfunctional in Cystic Fibrosis (CF) patients, and regulate platelet responses related to inflammation and its resolution. In order to further elucidate platelet involvement in CF inflammation, we pursued a comparative proteomic analysis of cells from healthy donors and CF patients, in association with a non-supervised comparative analysis of the Gene Ontology. Our results, showing changes in the integrin signalling in CF, support a pro-inflammatory profile of CF platelets.
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U2 - 10.1039/c0mb00135j
DO - 10.1039/c0mb00135j
M3 - Article
C2 - 21072441
AN - SCOPUS:79951593015
VL - 7
SP - 630
EP - 639
JO - Molecular BioSystems
JF - Molecular BioSystems
SN - 1742-206X
IS - 3
ER -