Proteomics investigation of human platelets in healthy donors and cystic fibrosis patients by shotgun nUPLC-MSE and 2DE: A comparative study

Luisa Pieroni; Francesco Finamore; Maurizio Ronci; Domenico Mattoscio; Valeria Marzano; Stefano Levi Mortera; Serena Quattrucci; Giorgio Federici; Mario Romano; Andrea Urbani

doi:10.1039/c0mb00135j

Proteomics investigation of human platelets in healthy donors and cystic fibrosis patients by shotgun nUPLC-MSE and 2DE: A comparative study

Luisa Pieroni, Francesco Finamore, Maurizio Ronci, Domenico Mattoscio, Valeria Marzano, Stefano Levi Mortera, Serena Quattrucci, Giorgio Federici, Mario Romano, Andrea Urbani

Research output: Contribution to journal › Article

Abstract

Platelets are of pathophysiological relevance in haemostasis, wound repair, inflammation and cardiovascular disease. We have shown that human platelets express a biologically active Cystic Fibrosis Transmembrane Conductance Regulator, which is dysfunctional in Cystic Fibrosis (CF) patients, and regulate platelet responses related to inflammation and its resolution. In order to further elucidate platelet involvement in CF inflammation, we pursued a comparative proteomic analysis of cells from healthy donors and CF patients, in association with a non-supervised comparative analysis of the Gene Ontology. Our results, showing changes in the integrin signalling in CF, support a pro-inflammatory profile of CF platelets.

Original language	English
Pages (from-to)	630-639
Number of pages	10
Journal	Molecular BioSystems
Volume	7
Issue number	3
DOIs	https://doi.org/10.1039/c0mb00135j
Publication status	Published - Mar 1 2011

ASJC Scopus subject areas

Biotechnology
Molecular Biology

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Pieroni, L., Finamore, F., Ronci, M., Mattoscio, D., Marzano, V., Mortera, S. L., Quattrucci, S., Federici, G., Romano, M., & Urbani, A. (2011). Proteomics investigation of human platelets in healthy donors and cystic fibrosis patients by shotgun nUPLC-MSE and 2DE: A comparative study. Molecular BioSystems, 7(3), 630-639. https://doi.org/10.1039/c0mb00135j

Proteomics investigation of human platelets in healthy donors and cystic fibrosis patients by shotgun nUPLC-MSE and 2DE : A comparative study. / Pieroni, Luisa; Finamore, Francesco; Ronci, Maurizio; Mattoscio, Domenico; Marzano, Valeria; Mortera, Stefano Levi; Quattrucci, Serena; Federici, Giorgio; Romano, Mario; Urbani, Andrea.

In: Molecular BioSystems, Vol. 7, No. 3, 01.03.2011, p. 630-639.

Research output: Contribution to journal › Article

Pieroni, Luisa ; Finamore, Francesco ; Ronci, Maurizio ; Mattoscio, Domenico ; Marzano, Valeria ; Mortera, Stefano Levi ; Quattrucci, Serena ; Federici, Giorgio ; Romano, Mario ; Urbani, Andrea. / Proteomics investigation of human platelets in healthy donors and cystic fibrosis patients by shotgun nUPLC-MSE and 2DE : A comparative study. In: Molecular BioSystems. 2011 ; Vol. 7, No. 3. pp. 630-639.

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