Proteomics investigation of human platelets in healthy donors and cystic fibrosis patients by shotgun nUPLC-MSE and 2DE: A comparative study

Luisa Pieroni, Francesco Finamore, Maurizio Ronci, Domenico Mattoscio, Valeria Marzano, Stefano Levi Mortera, Serena Quattrucci, Giorgio Federici, Mario Romano, Andrea Urbani

Research output: Contribution to journalArticle

Abstract

Platelets are of pathophysiological relevance in haemostasis, wound repair, inflammation and cardiovascular disease. We have shown that human platelets express a biologically active Cystic Fibrosis Transmembrane Conductance Regulator, which is dysfunctional in Cystic Fibrosis (CF) patients, and regulate platelet responses related to inflammation and its resolution. In order to further elucidate platelet involvement in CF inflammation, we pursued a comparative proteomic analysis of cells from healthy donors and CF patients, in association with a non-supervised comparative analysis of the Gene Ontology. Our results, showing changes in the integrin signalling in CF, support a pro-inflammatory profile of CF platelets.

Original languageEnglish
Pages (from-to)630-639
Number of pages10
JournalMolecular BioSystems
Volume7
Issue number3
DOIs
Publication statusPublished - Mar 1 2011

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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