Proteomics meets genetics

SILAC labeling of drosophila melanogaster larvae and cells for in vivo functional studies

Alessandro Cuomo, Roberta Sanfilippo, Thomas Vaccari, Tiziana Bonaldi

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Stable isotope labeling by amino acids in cell culture (SILAC) is an established and potent method for quantitative proteomics. When combined with high-resolution mass spectrometry (MS) and efficient algorithms for the analysis of quantitative MS data, SILAC has proven to be the strategy of choice for the in-depth characterization of functional states at the protein level. The fruit fly Drosophila melanogaster is one of the most widely used model systems for studies of genetics and developmental biology. Despite this, a global proteomic approach in Drosophila is rarely considered. Here, we describe an adaptation of SILAC for functional investigation of fruit flies by proteomics: We illustrate how to perform efficient SILAC labeling of cells in culture and whole fly larvae. The combination of SILAC, a highly accurate global protein quantification method, and of the fruit fly, the prime genetics and developmental model, represents a unique opportunity for quantitative proteomic studies in vivo.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages293-311
Number of pages19
Volume1188
ISBN (Print)9781493911417
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1188
ISSN (Print)10643745

Fingerprint

Drosophila melanogaster
Diptera
Proteomics
Larva
Fruit
Mass Spectrometry
Cell Culture Techniques
Isotope Labeling
Developmental Biology
Genetic Models
Drosophila
Proteins
Amino Acids

Keywords

  • Drosophila melanogaster
  • Intact organism labeling
  • Mass spectrometry
  • Quantitative proteomics
  • Saccharomyces cerevisiae
  • Schneider cell
  • SILAC

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Cuomo, A., Sanfilippo, R., Vaccari, T., & Bonaldi, T. (2014). Proteomics meets genetics: SILAC labeling of drosophila melanogaster larvae and cells for in vivo functional studies. In Methods in Molecular Biology (Vol. 1188, pp. 293-311). (Methods in Molecular Biology; Vol. 1188). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-1142-4_21

Proteomics meets genetics : SILAC labeling of drosophila melanogaster larvae and cells for in vivo functional studies. / Cuomo, Alessandro; Sanfilippo, Roberta; Vaccari, Thomas; Bonaldi, Tiziana.

Methods in Molecular Biology. Vol. 1188 Humana Press Inc., 2014. p. 293-311 (Methods in Molecular Biology; Vol. 1188).

Research output: Chapter in Book/Report/Conference proceedingChapter

Cuomo, A, Sanfilippo, R, Vaccari, T & Bonaldi, T 2014, Proteomics meets genetics: SILAC labeling of drosophila melanogaster larvae and cells for in vivo functional studies. in Methods in Molecular Biology. vol. 1188, Methods in Molecular Biology, vol. 1188, Humana Press Inc., pp. 293-311. https://doi.org/10.1007/978-1-4939-1142-4_21
Cuomo A, Sanfilippo R, Vaccari T, Bonaldi T. Proteomics meets genetics: SILAC labeling of drosophila melanogaster larvae and cells for in vivo functional studies. In Methods in Molecular Biology. Vol. 1188. Humana Press Inc. 2014. p. 293-311. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-1142-4_21
Cuomo, Alessandro ; Sanfilippo, Roberta ; Vaccari, Thomas ; Bonaldi, Tiziana. / Proteomics meets genetics : SILAC labeling of drosophila melanogaster larvae and cells for in vivo functional studies. Methods in Molecular Biology. Vol. 1188 Humana Press Inc., 2014. pp. 293-311 (Methods in Molecular Biology).
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