Proteomics of inflammatory and oxidative stress response in cows with subclinical and clinical mastitis

Romana Turk, Cristian Piras, Mislav Kovačić, Marko Samardžija, Hany Ahmed, Michele De Canio, Andrea Urbani, Zlata Flegar Meštrić, Alessio Soggiu, Luigi Bonizzi, Paola Roncada

Research output: Contribution to journalArticle

Abstract

Cow serum proteome was evaluated by three different complementary approaches in the control group, subclinical and clinical mastitis in order to possibly find differential protein expression useful for a better understanding of the pathophysiology of mastitis as well as for an early diagnosis of the disease. The systemic inflammatory and oxidative stress response in cows with subclinical and clinical mastitis were observed. The collected evidence shows a differential protein expression of serpin A3-1, vitronectin-like protein and complement factor H in subclinical mastitis in comparison with the control. It was also found a differential protein expression of inter-alpha-trypsin inhibitor heavy chain H4, serpin A3-1, C4b-binding protein alpha chain, haptoglobin and apolipoprotein A-I in clinical mastitis compared to the control. Among the inflammatory proteins up-regulated in clinical mastitis, vitronectin is over-expressed in both subclinical and clinical mastitis indicating a strong bacterial infection. This suggests vitronectin as an important mediator in the pathogenesis of the onset of mastitis as well as a valuable marker for diagnosis of the subclinical form of the disease. Obtained data could be useful for the detection of mastitis during the subclinical phase and for a better comprehension of the pathophysiological mechanisms involved in the onset of the disease. This article is part of a Special Issue entitled: Farm animal proteomics. This article is part of a Special Issue entitled: Farm animal proteomics.

Original languageEnglish
Pages (from-to)4412-4428
Number of pages17
JournalJournal of Proteomics
Volume75
Issue number14
DOIs
Publication statusPublished - Jul 19 2012

Keywords

  • Cows
  • Inflammation
  • Mastitis
  • Oxidative stress
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Fingerprint Dive into the research topics of 'Proteomics of inflammatory and oxidative stress response in cows with subclinical and clinical mastitis'. Together they form a unique fingerprint.

  • Cite this

    Turk, R., Piras, C., Kovačić, M., Samardžija, M., Ahmed, H., De Canio, M., Urbani, A., Meštrić, Z. F., Soggiu, A., Bonizzi, L., & Roncada, P. (2012). Proteomics of inflammatory and oxidative stress response in cows with subclinical and clinical mastitis. Journal of Proteomics, 75(14), 4412-4428. https://doi.org/10.1016/j.jprot.2012.05.021