TY - JOUR
T1 - Proteomics of rat biological fluids - The tenth anniversary update
AU - Gianazza, Elisabetta
AU - Wait, Robin
AU - Eberini, Ivano
AU - Sensi, Cristina
AU - Sironi, Luigi
AU - Miller, Ingrid
PY - 2012/6/18
Y1 - 2012/6/18
N2 - In addition to a remarkable sexual dimorphism of serum and urine proteomes, the rat is exceptional for the wide difference between the serum patterns during an acute phase reaction vs baseline conditions. This feature allows monitoring with high sensitivity onset and progression of any pathological state that involves an inflammatory component as well as assessing the outcome of any therapeutic intervention.Reference maps have been defined for the proteomes of serum, urine, cerebrospinal fluid and bronchoalveolar lavage fluid. For both serum and urine most of the proteomic investigations have dealt with toxicological testing, for BALF with allergic or irritative reactions, whereas with CSF the main aim was the characterization of rat models of neurological disorders.When surveying more than ten years of literature on rat biological fluid proteomics, it is puzzling to see how seldom a consistent analytical plan has been set up for the comparative investigation on two or more types of sample, whether to fully characterize a disease model or to evaluate pharmacological/toxicological effects of a drug. It is also regrettable that in several cases only a negligible part of the results is discussed at length whereas most data are not even made known to the scientific community.
AB - In addition to a remarkable sexual dimorphism of serum and urine proteomes, the rat is exceptional for the wide difference between the serum patterns during an acute phase reaction vs baseline conditions. This feature allows monitoring with high sensitivity onset and progression of any pathological state that involves an inflammatory component as well as assessing the outcome of any therapeutic intervention.Reference maps have been defined for the proteomes of serum, urine, cerebrospinal fluid and bronchoalveolar lavage fluid. For both serum and urine most of the proteomic investigations have dealt with toxicological testing, for BALF with allergic or irritative reactions, whereas with CSF the main aim was the characterization of rat models of neurological disorders.When surveying more than ten years of literature on rat biological fluid proteomics, it is puzzling to see how seldom a consistent analytical plan has been set up for the comparative investigation on two or more types of sample, whether to fully characterize a disease model or to evaluate pharmacological/toxicological effects of a drug. It is also regrettable that in several cases only a negligible part of the results is discussed at length whereas most data are not even made known to the scientific community.
KW - Animal models of disease
KW - Broncho-alveolar lavage fluid
KW - Cerebrospinal fluid
KW - Serum
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=84861687257&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861687257&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2012.03.053
DO - 10.1016/j.jprot.2012.03.053
M3 - Article
C2 - 22504499
AN - SCOPUS:84861687257
VL - 75
SP - 3113
EP - 3128
JO - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
IS - 11
ER -