Proteomics of the acid-soluble fraction of whole and major gland saliva in burning mouth syndrome patients

Tiziana Cabras, Barbara Manconi, Massimo Castagnola, Maria Teresa Sanna, Morena Arba, Shikha Acharya, Jörgen Ekström, Anette Carlén, Irene Messana

Research output: Contribution to journalArticlepeer-review


OBJECTIVE: In the present study the salivary proteome of burning mouth syndrome patients and healthy subjects was characterized by a top-down proteomic approach and compared to highlight possible qualitative and quantitative differences that may give suggestions about the causes of this pathology which are still unknown.

MATERIALS AND METHODS: Resting and stimulated whole saliva, stimulated parotid and submandibular/sublingual saliva samples were collected from burning mouth syndrome patients (n = 16) and age- and gender-matched healthy subjects (n = 14). An equal volume of 0.2% trifluoroacetic acid was added to each sample immediately after collection and the supernatants were analysed by liquid chromatography coupled to electrospray-ionisation mass spectrometry. Proteins and peptides were quantified using a label-free approach measuring the extracted ion current peak areas of the main salivary proteins and peptides.

RESULTS: The quantitation of the main salivary proteins and peptides revealed a higher concentration of cystatin SN in resting saliva of burning mouth syndrome patients with respect to healthy controls and no other conspicuous changes.

CONCLUSIONS: The reported data showed that the salivary protein profile was not affected, in composition and relative abundance, by the burning mouth syndrome, except for the cystatin SN, a protein up-regulated in several pathological conditions, that might be considered potentially indicative of the disease.

Original languageEnglish
Pages (from-to)148-155
Number of pages8
JournalArchives of Oral Biology
Publication statusPublished - Feb 2019


  • Aged
  • Burning Mouth Syndrome/complications
  • Chromatography, Liquid
  • Female
  • Humans
  • Middle Aged
  • Parotid Gland/metabolism
  • Proteome/metabolism
  • Proteomics/methods
  • Saliva/chemistry
  • Salivary Glands/chemistry
  • Salivary Proteins and Peptides/analysis
  • Salivation
  • Spectrometry, Mass, Electrospray Ionization
  • Xerostomia/complications

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