Proteomineering: Has the mine been excavated?

Isabella Panfoli, Maurizio Bruschi, Silvia Ravera, Giovanni Candiano

Research output: Contribution to journalArticle

Abstract

The combinatorial peptide ligand library in association with mass spectrometry can greatly enhance the dynamic range of the analysis of low- and very low-abundance proteins constituting the vast majority of species in any sample. When compared with untreated samples, the increment in detection of low-abundance species appears to be at least fourfold. Recently, the combinatorial peptide ligand library has been challenged; however, it has been clearly demonstrated in the evaluated paper that the protocols for elution of the captured polypeptides make the difference. Therefore, the solid-phase ligand library made of hexapeptides remains a promising and unique tool for biomarker discovery.

Original languageEnglish
Pages (from-to)443-445
Number of pages3
JournalExpert Review of Proteomics
Volume8
Issue number4
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Peptide Library
Ligands
Peptides
Library Associations
Biomarkers
Mass spectrometry
Mass Spectrometry
Association reactions
Proteins

Keywords

  • biomarkers
  • combinatorial peptide libraries
  • elution protocols
  • hexapeptide ligands
  • low-abundance proteome
  • mass spectrometry

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Proteomineering : Has the mine been excavated? / Panfoli, Isabella; Bruschi, Maurizio; Ravera, Silvia; Candiano, Giovanni.

In: Expert Review of Proteomics, Vol. 8, No. 4, 08.2011, p. 443-445.

Research output: Contribution to journalArticle

Panfoli, Isabella ; Bruschi, Maurizio ; Ravera, Silvia ; Candiano, Giovanni. / Proteomineering : Has the mine been excavated?. In: Expert Review of Proteomics. 2011 ; Vol. 8, No. 4. pp. 443-445.
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