Prothrombotic effects of fibronectin isoforms containing the EDA domain

Anil K. Chauhan, Janka Kisucka, Maria R. Cozzi, Meghan T. Walsh, Federico A. Moretti, Monica Battiston, Mario Mazzucato, Luigi De Marco, Francisco E. Baralle, Denisa D. Wagner, Andrés F. Muro

Research output: Contribution to journalArticle

Abstract

OBJECTIVE - Fibronectin (FN) plays an important role in the formation of stable arterial thrombi at the site of vascular injury. FN containing Extra Domain A (EDAFN) is absent from normal plasma, but elevated plasma levels of EDAFN are found in several pathological conditions. We hypothesized that EDAFN plays a special role in thrombosis. METHODS AND RESULTS - We used mouse strains constitutively including (EDA) or excluding (EDA) the EDA domain in all tissues and plasma. Using a flow chamber and the ferric-chloride injury model we found that EDAFN accelerates thrombosis both in vitro and in vivo at arterial shear rates. In EDA mice thrombi (>30 μm) grew faster when compared with EDA (6.6±0.2 minutes versus 8.3±0.6 minutes, P

Original languageEnglish
Pages (from-to)296-301
Number of pages6
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume28
Issue number2
DOIs
Publication statusPublished - Feb 2008

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Keywords

  • Arterial and venous injury
  • Arterial thrombosis
  • Fibronectin splice variants isoforms
  • Intravital microscopy
  • Plasma fibronectin
  • Thromboembolism

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Chauhan, A. K., Kisucka, J., Cozzi, M. R., Walsh, M. T., Moretti, F. A., Battiston, M., Mazzucato, M., De Marco, L., Baralle, F. E., Wagner, D. D., & Muro, A. F. (2008). Prothrombotic effects of fibronectin isoforms containing the EDA domain. Arteriosclerosis, Thrombosis, and Vascular Biology, 28(2), 296-301. https://doi.org/10.1161/ATVBAHA.107.149146