Prothymosin alpha protects cardiomyocytes against ischemia-induced apoptosis via preservation of Akt activation

Alessandro Cannavo, Giuseppe Rengo, Daniela Liccardo, Gianluigi Pironti, Maria Cecilia Scimia, Laura Scudiero, Claudio De Lucia, Marco Ferrone, Dario Leosco, Nicola Zambrano, Walter J. Koch, Bruno Trimarco, Giovanni Esposito

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The human prothymosin alpha (PTα) gene encodes a 12.5 kDa highly acidic nuclear protein that is widely expressed in mammalian tissues including the heart and importantly, is detectable also in blood serum. During apoptosis or necrosis, PTα changes its nuclear localization and is able to exert an important cytoprotective effect. Since the role of PTα in the heart has never been evaluated, the aim of the present study was to investigate the effects of PTα on cardiomyocytes during ischemic injury. Our data show that seven after myocardial infarction (MI), PTα expression levels are significantly increased both in blood serum and in cardiac tissue, and notably we observe that PTα translocates from the nuclei to cytoplasm and plasma membrane of cardiomyocytes following MI. Furthermore, in vitro experiments in cardiomyocytes, confirm that after 6 h of simulated ischemia (SI), PTα protein levels are upregulated compared to normoxic cells. Importantly, treatment of cardiomyocytes with a recombinant PTα (rPTα), during SI results in a significant decrease in the apoptotic response and in a robust increase in cell survival. Moreover, these effects are accompanied to a significant preservation of the activated levels of the anti-apoptotic serine-threonine kinase Akt. Consistent with our in vitro observation, rPTα-treated MI mice exhibit a strong reduction in infarct size at 24 h, compared to the MI control group and at the molecular level, PTα treatment induces activation of Akt. The present study provides for the first time the demonstration that PTα offers cardioprotection against ischemic injury by an Akt-dependent mechanism.

Original languageEnglish
Pages (from-to)1252-1261
Number of pages10
JournalApoptosis
Volume18
Issue number10
DOIs
Publication statusPublished - 2013

Fingerprint

Cardiac Myocytes
Ischemia
Chemical activation
Apoptosis
Myocardial Infarction
Blood
prothymosin alpha
Tissue
Protein-Serine-Threonine Kinases
Wounds and Injuries
Cell membranes
Nuclear Proteins
Serum
Cell Survival
Cytoplasm
Necrosis
Demonstrations
Genes
Cells
Cell Membrane

Keywords

  • Apoptosis
  • Cardioprotection
  • Ischemia
  • Prothymosin alpha

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Biochemistry, medical
  • Cancer Research
  • Pharmaceutical Science
  • Pharmacology

Cite this

Cannavo, A., Rengo, G., Liccardo, D., Pironti, G., Scimia, M. C., Scudiero, L., ... Esposito, G. (2013). Prothymosin alpha protects cardiomyocytes against ischemia-induced apoptosis via preservation of Akt activation. Apoptosis, 18(10), 1252-1261. https://doi.org/10.1007/s10495-013-0876-9

Prothymosin alpha protects cardiomyocytes against ischemia-induced apoptosis via preservation of Akt activation. / Cannavo, Alessandro; Rengo, Giuseppe; Liccardo, Daniela; Pironti, Gianluigi; Scimia, Maria Cecilia; Scudiero, Laura; De Lucia, Claudio; Ferrone, Marco; Leosco, Dario; Zambrano, Nicola; Koch, Walter J.; Trimarco, Bruno; Esposito, Giovanni.

In: Apoptosis, Vol. 18, No. 10, 2013, p. 1252-1261.

Research output: Contribution to journalArticle

Cannavo, A, Rengo, G, Liccardo, D, Pironti, G, Scimia, MC, Scudiero, L, De Lucia, C, Ferrone, M, Leosco, D, Zambrano, N, Koch, WJ, Trimarco, B & Esposito, G 2013, 'Prothymosin alpha protects cardiomyocytes against ischemia-induced apoptosis via preservation of Akt activation', Apoptosis, vol. 18, no. 10, pp. 1252-1261. https://doi.org/10.1007/s10495-013-0876-9
Cannavo, Alessandro ; Rengo, Giuseppe ; Liccardo, Daniela ; Pironti, Gianluigi ; Scimia, Maria Cecilia ; Scudiero, Laura ; De Lucia, Claudio ; Ferrone, Marco ; Leosco, Dario ; Zambrano, Nicola ; Koch, Walter J. ; Trimarco, Bruno ; Esposito, Giovanni. / Prothymosin alpha protects cardiomyocytes against ischemia-induced apoptosis via preservation of Akt activation. In: Apoptosis. 2013 ; Vol. 18, No. 10. pp. 1252-1261.
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