Protocatechuic acid activates key components of insulin signaling pathway mimicking insulin activity

Beatrice Scazzocchio, Rosaria Varì, Carmelina Filesi, Ilaria Del Gaudio, Massimo D'Archivio, Carmela Santangelo, Annunziata Iacovelli, Fabio Galvano, Francesca Romana Pluchinotta, Claudio Giovannini, Roberta Masella

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Scope: Insulin resistance represents an independent risk factor for metabolic and cardiovascular diseases. Researchers have been interested in identifying active harmless compounds, as many insulin-sensitizing drugs have shown unwanted side-effects. It has been demonstrated that anthocyanins and one of their representative metabolites, protocatechuic acid (PCA), ameliorate hyperglycemia, and insulin sensitivity. This study investigated the mechanism of action of PCA responsible for the glucose uptake upregulation. Methods and results: In human visceral adipocytes, PCA stimulated insulin receptor substrate-1 (IRS-1) tyrosine phosphorylation (+40% with respect to untreated cells) and the downstream events, i.e. phosphoinositide 3-kinase binding to IRS-1 and Akt phosphorylation (+100%, +180%, respectively, with respect to untreated cells). The insulin-like activity of PCA seemed to be mediated by insulin receptor since by inhibiting its autophosphorylation, the PCA effects were completely abolished. Furthermore, PCA was able to activate adenosine monophosphate-activated protein kinase, a serine/threonine kinase whose activation elicits insulin-sensitizing effects. Conclusion: This study showed that PCA stimulates the insulin signaling pathway in human adipocytes increasing GLUT4 translocation and glucose uptake. Decreasing insulin resistance is a most desirable aim to be reached for an effective therapeutic/preventive action against metabolic syndrome and type 2 diabetes. Identifying specific food/food components able to improve glucose metabolism can offer an attractive, novel, and economical strategy.

Original languageEnglish
Pages (from-to)1472-1481
Number of pages10
JournalMolecular Nutrition and Food Research
Volume59
Issue number8
DOIs
Publication statusPublished - Aug 1 2015

Fingerprint

protocatechuic acid
insulin
Insulin
insulin resistance
Insulin Resistance
Insulin Receptor Substrate Proteins
adipocytes
Adipocytes
Glucose
glucose
phosphorylation
insulin-like effect
Phosphorylation
uptake mechanisms
Food
AMP-activated protein kinase
1-Phosphatidylinositol 4-Kinase
Anthocyanins
Protein-Serine-Threonine Kinases
protein phosphorylation

Keywords

  • Adipocytes
  • Diet
  • Functional food
  • Insulin resistance
  • Insulin signaling

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

Cite this

Scazzocchio, B., Varì, R., Filesi, C., Del Gaudio, I., D'Archivio, M., Santangelo, C., ... Masella, R. (2015). Protocatechuic acid activates key components of insulin signaling pathway mimicking insulin activity. Molecular Nutrition and Food Research, 59(8), 1472-1481. https://doi.org/10.1002/mnfr.201400816

Protocatechuic acid activates key components of insulin signaling pathway mimicking insulin activity. / Scazzocchio, Beatrice; Varì, Rosaria; Filesi, Carmelina; Del Gaudio, Ilaria; D'Archivio, Massimo; Santangelo, Carmela; Iacovelli, Annunziata; Galvano, Fabio; Pluchinotta, Francesca Romana; Giovannini, Claudio; Masella, Roberta.

In: Molecular Nutrition and Food Research, Vol. 59, No. 8, 01.08.2015, p. 1472-1481.

Research output: Contribution to journalArticle

Scazzocchio, B, Varì, R, Filesi, C, Del Gaudio, I, D'Archivio, M, Santangelo, C, Iacovelli, A, Galvano, F, Pluchinotta, FR, Giovannini, C & Masella, R 2015, 'Protocatechuic acid activates key components of insulin signaling pathway mimicking insulin activity', Molecular Nutrition and Food Research, vol. 59, no. 8, pp. 1472-1481. https://doi.org/10.1002/mnfr.201400816
Scazzocchio, Beatrice ; Varì, Rosaria ; Filesi, Carmelina ; Del Gaudio, Ilaria ; D'Archivio, Massimo ; Santangelo, Carmela ; Iacovelli, Annunziata ; Galvano, Fabio ; Pluchinotta, Francesca Romana ; Giovannini, Claudio ; Masella, Roberta. / Protocatechuic acid activates key components of insulin signaling pathway mimicking insulin activity. In: Molecular Nutrition and Food Research. 2015 ; Vol. 59, No. 8. pp. 1472-1481.
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