Protocols for developing and testing methods applied to the quality control of botanicals

Mihaela Badea, Concetta Boniglia, Brunella Carratù, Remigius Chizzola, Chlodwig Franz, Stefania Giammarioli, Maurizio Mosca, Johannes Novak, Paul A. Steenkamp, Patrizia Restani

Research output: Chapter in Book/Report/Conference proceedingChapter


Both the application of official/published methods and the development of a novel analytical protocol need specific guidelines for their validation. Methods developed for quality controls of botanicals could be classified as: (1) phytochemical assays, and DNA-based identification; (2) methods for the identification and quantification of specific compounds. The validation of the methods for phytochemical and DNA-based identification requires the following parameters: ruggedness, specificity and limit of detection (LOD). In relation to development and testing of methods for detection of specific compounds: beneficial and toxic substances, contaminants, residues and biomarkers, this chapter presents guidelines based on good analytical practices, to assist analyst in obtaining data of requested quality and to aid in the evaluation of the quality of obtained data. This chapter will be organised in the following three parts: (1) evaluation of the problem and characterization of analytical requirements; (2) development of methods; and (3) validation of methods. The parameters that have to be evaluated for in-house validation will be different depending on the type of method (qualitative or quantitative, compounds in high concentration or traces).

Original languageEnglish
Title of host publicationFood Supplements Containing Botanicals
Subtitle of host publicationBenefits, Side Effects and Regulatory Aspects: The Scientific Inheritance of the EU Project PlantLIBRA
PublisherSpringer International Publishing AG
Number of pages12
ISBN (Electronic)9783319622293
ISBN (Print)9783319622286
Publication statusPublished - Jan 1 2017


  • Adulterations
  • Botanical fingerprints
  • Contaminants
  • Quality control

ASJC Scopus subject areas

  • Engineering(all)
  • Agricultural and Biological Sciences(all)


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