Proton inactivation of melanoma cells enhanced by fotemustine

Aleksandra Ristić-Fira, Lela Korićanac, Jelena Žakula, Otilija Keta, Gioacchin Iannolo, Giacomo Cuttone, Ivan Petrović

Research output: Contribution to journalArticlepeer-review


Response of human HTB140 melanoma cells to proton irradiation in combination with fotemustine (FM) was investigated. Effects of these agents were analysed on cell proliferation and induction of apoptosis. Cells pretreated with 100- or 250-μM of FM were irradiated in the middle of the therapeutic 62-MeV proton spread-out Bragg peak, with a dose of 16 Gy. All treatments reduced proliferation and survival of melanoma cells. The most pronounced effects of the combined treatment were obtained for cell survivals. The level of apoptosis increased after all applied treatments. Particularly good pro-apoptotic effect was achieved when proton irradiation was combined with 250 μM of FM. This was followed by the increased expression of p53 gene. The obtained results have shown that combined application of FM and protons significantly reduced growth of this resistant melanoma cell line.

Original languageEnglish
Article numberncq527
Pages (from-to)503-507
Number of pages5
JournalRadiation Protection Dosimetry
Issue number2-4
Publication statusPublished - Feb 2011

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Radiation
  • Public Health, Environmental and Occupational Health


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