Protonation curves of the D10N variant of endoglucanase Cel45 catalytic domain from Humicola insolens: Isothermal calorimetry and combined isoelectric focusing-electrophoresis

F. Attanasio, M. Bruschi, G. Candiano, R. Ponassi, N. Castagnino, M. Schülein, G. Rialdi

Research output: Contribution to journalArticlepeer-review

Abstract

Titration of ionizable groups of D10N variant catalytic domain has been performed by isothermal calorimetric technique and by combined isoelectric focusing-electrophoresis in the range of pH 3-9. pK°i and ΔH°i related to the ionizable groups have been estimated using Lindestrøm-Lang equation. Both experimental techniques are in agreement and confirm the validity of the applied theory. Slight differences in protonation enthalpies of carboxyl groups are caused by Asp10 interaction with the other part of the macromolecule.

Original languageEnglish
Pages (from-to)123-132
Number of pages10
JournalJournal of Thermal Analysis and Calorimetry
Volume66
Issue number1
DOIs
Publication statusPublished - 2001

Keywords

  • Cel45
  • Protein isoelectric focusing electrophoresis
  • Protein isothermal calorimetry
  • Protonation heat

ASJC Scopus subject areas

  • Chemical Engineering(all)

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