Protonation curves of the D10N variant of endoglucanase Cel45 catalytic domain from Humicola insolens: Isothermal calorimetry and combined isoelectric focusing-electrophoresis

F. Attanasio; M. Bruschi; G. Candiano; R. Ponassi; N. Castagnino; M. Schülein; G. Rialdi

doi:10.1023/A:1012439732130

Protonation curves of the D10N variant of endoglucanase Cel45 catalytic domain from Humicola insolens: Isothermal calorimetry and combined isoelectric focusing-electrophoresis

F. Attanasio, M. Bruschi, G. Candiano, R. Ponassi, N. Castagnino, M. Schülein, G. Rialdi

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article › peer-review

Abstract

Titration of ionizable groups of D10N variant catalytic domain has been performed by isothermal calorimetric technique and by combined isoelectric focusing-electrophoresis in the range of pH 3-9. pK°_i and ΔH°_i related to the ionizable groups have been estimated using Lindestrøm-Lang equation. Both experimental techniques are in agreement and confirm the validity of the applied theory. Slight differences in protonation enthalpies of carboxyl groups are caused by Asp10 interaction with the other part of the macromolecule.

Original language	English
Pages (from-to)	123-132
Number of pages	10
Journal	Journal of Thermal Analysis and Calorimetry
Volume	66
Issue number	1
DOIs	https://doi.org/10.1023/A:1012439732130
Publication status	Published - 2001

Keywords

Cel45
Protein isoelectric focusing electrophoresis
Protein isothermal calorimetry
Protonation heat

ASJC Scopus subject areas

Chemical Engineering(all)

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Attanasio, F., Bruschi, M., Candiano, G., Ponassi, R., Castagnino, N., Schülein, M., & Rialdi, G. (2001). Protonation curves of the D10N variant of endoglucanase Cel45 catalytic domain from Humicola insolens: Isothermal calorimetry and combined isoelectric focusing-electrophoresis. Journal of Thermal Analysis and Calorimetry, 66(1), 123-132. https://doi.org/10.1023/A:1012439732130

Protonation curves of the D10N variant of endoglucanase Cel45 catalytic domain from Humicola insolens : Isothermal calorimetry and combined isoelectric focusing-electrophoresis. / Attanasio, F.; Bruschi, M.; Candiano, G.; Ponassi, R.; Castagnino, N.; Schülein, M.; Rialdi, G.

In: Journal of Thermal Analysis and Calorimetry, Vol. 66, No. 1, 2001, p. 123-132.

Research output: Contribution to journal › Article › peer-review

Attanasio, F, Bruschi, M, Candiano, G, Ponassi, R, Castagnino, N, Schülein, M & Rialdi, G 2001, 'Protonation curves of the D10N variant of endoglucanase Cel45 catalytic domain from Humicola insolens: Isothermal calorimetry and combined isoelectric focusing-electrophoresis', Journal of Thermal Analysis and Calorimetry, vol. 66, no. 1, pp. 123-132. https://doi.org/10.1023/A:1012439732130

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abstract = "Titration of ionizable groups of D10N variant catalytic domain has been performed by isothermal calorimetric technique and by combined isoelectric focusing-electrophoresis in the range of pH 3-9. pK°i and ΔH°i related to the ionizable groups have been estimated using Lindestr{\o}m-Lang equation. Both experimental techniques are in agreement and confirm the validity of the applied theory. Slight differences in protonation enthalpies of carboxyl groups are caused by Asp10 interaction with the other part of the macromolecule.",

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AU - Schülein, M.

AU - Rialdi, G.

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KW - Protein isoelectric focusing electrophoresis

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KW - Protonation heat

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