Protozoan and hepatitis B virus DNA detection in human samples

Laura Biagiotti, Maurizio Gramegna, Massimiliano Valisi, Stefania Madama, Giuliana Saltini, Roberto Dominici, Giulia Malferrari, Eugenia Monferini, Maria Carla Proverbio, Pasquale De Blasio, Ida Biunno

Research output: Contribution to journalArticle

Abstract

Polymerase chain reaction (PCR) amplification of a marker sequence is a convenient and practical tool in epidemiological studies, where only limited amounts of specimens, such as blood, are available. Methods for DMA preparation from blood samples in studies of epidemiological scale have to be simple, efficient, rapid to prepare, versatile, suitable for large-scale screening, and inexpensive. The quality and quantity of DNA extracted, with slight modifications of the original procedure, enabled the detection of protozoan particles in infected human whole blood and Leishmania in cultured cells.

Original languageEnglish
Pages (from-to)681-683
Number of pages3
JournalLaboratory Medicine
Volume35
Issue number11
DOIs
Publication statusPublished - Nov 2004

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Pathology and Forensic Medicine

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  • Cite this

    Biagiotti, L., Gramegna, M., Valisi, M., Madama, S., Saltini, G., Dominici, R., Malferrari, G., Monferini, E., Proverbio, M. C., De Blasio, P., & Biunno, I. (2004). Protozoan and hepatitis B virus DNA detection in human samples. Laboratory Medicine, 35(11), 681-683. https://doi.org/10.1309/6G40-CDUU-CW2W-JFR6