Polymerase chain reaction (PCR) amplification of a marker sequence is a convenient and practical tool in epidemiological studies, where only limited amounts of specimens, such as blood, are available. Methods for DMA preparation from blood samples in studies of epidemiological scale have to be simple, efficient, rapid to prepare, versatile, suitable for large-scale screening, and inexpensive. The quality and quantity of DNA extracted, with slight modifications of the original procedure, enabled the detection of protozoan particles in infected human whole blood and Leishmania in cultured cells.
ASJC Scopus subject areas
- Clinical Biochemistry
- Pathology and Forensic Medicine