Protozoi patogeni per l'uomo

Protocolli di caratterizzazione differenziale di genere e di specie nell'ambito del complesso Entamoeba histolytica/Entamoeba dispar, Acanthamoeba spp., Microsporidi

Massimo Scaglia, S. Gatti, A. Bruno, A. M. Bernuzzi, C. Cevini, R. Maserati

Research output: Contribution to journalArticle

Abstract

The review summarizes the results in the main parasitological topics of our Lab: amoebic infections due to Entamoeba histolytica/Entamoeba dispar complex and to Acanthamoeba spp. respectively, and human infections caused by microsporidia. Different rapid and advanced techniques have been included in the standardized diagnostic protocols for each topic, and a critical comparison among them was made, in order to define the gold standard diagnostic method: a) E. histolytica/E. dispar: in vitro culture, zymodeme typization, biomolecular identification (PCR), immunoenzymatic assay (ELISA) for direct detection in stools of specific surface antigenic lectins; b) Acanthamoeba spp.: In vitro culture, light and ultrastructural characterization, species identification by immunofluorescence method with monoclonal antibodies, in vitro pharmacological studies; c) Microsporidia: ultrastructural (TEM), biomolecular (PCR), biochemical and immunological (SDS-PAGE, Immunoblotting) studies for species identification, use of advanced ultrastructural techniques ("freeze-etching", "deep-etching") in order to deepen the spore wall structure, to study the cytoskeletal function of actin and to define the mode of infection, in vitro pharmacological assays on some inhibitors of chitin-synthases.

Original languageEnglish
Pages (from-to)37-44
Number of pages8
JournalParassitologia
Volume43
Issue numberSUPPL. 1
Publication statusPublished - 2001

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Entamoeba
Acanthamoeba
Entamoeba histolytica
Microsporidia
Infection
Chitin Synthase
Freeze Etching
Pharmacology
Polymerase Chain Reaction
Spores
Immunoblotting
Lectins
Fluorescent Antibody Technique
Actins
Polyacrylamide Gel Electrophoresis
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Light
In Vitro Techniques

Keywords

  • Acanthamoeba spp
  • Advanced diagnostic protocols
  • E. Histolytica/E. Dispar
  • Microsporidia

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

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title = "Protozoi patogeni per l'uomo: Protocolli di caratterizzazione differenziale di genere e di specie nell'ambito del complesso Entamoeba histolytica/Entamoeba dispar, Acanthamoeba spp., Microsporidi",
abstract = "The review summarizes the results in the main parasitological topics of our Lab: amoebic infections due to Entamoeba histolytica/Entamoeba dispar complex and to Acanthamoeba spp. respectively, and human infections caused by microsporidia. Different rapid and advanced techniques have been included in the standardized diagnostic protocols for each topic, and a critical comparison among them was made, in order to define the gold standard diagnostic method: a) E. histolytica/E. dispar: in vitro culture, zymodeme typization, biomolecular identification (PCR), immunoenzymatic assay (ELISA) for direct detection in stools of specific surface antigenic lectins; b) Acanthamoeba spp.: In vitro culture, light and ultrastructural characterization, species identification by immunofluorescence method with monoclonal antibodies, in vitro pharmacological studies; c) Microsporidia: ultrastructural (TEM), biomolecular (PCR), biochemical and immunological (SDS-PAGE, Immunoblotting) studies for species identification, use of advanced ultrastructural techniques ({"}freeze-etching{"}, {"}deep-etching{"}) in order to deepen the spore wall structure, to study the cytoskeletal function of actin and to define the mode of infection, in vitro pharmacological assays on some inhibitors of chitin-synthases.",
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AU - Scaglia, Massimo

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AU - Bruno, A.

AU - Bernuzzi, A. M.

AU - Cevini, C.

AU - Maserati, R.

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