Abstract
The review summarizes the results in the main parasitological topics of our Lab: amoebic infections due to Entamoeba histolytica/Entamoeba dispar complex and to Acanthamoeba spp. respectively, and human infections caused by microsporidia. Different rapid and advanced techniques have been included in the standardized diagnostic protocols for each topic, and a critical comparison among them was made, in order to define the gold standard diagnostic method: a) E. histolytica/E. dispar: in vitro culture, zymodeme typization, biomolecular identification (PCR), immunoenzymatic assay (ELISA) for direct detection in stools of specific surface antigenic lectins; b) Acanthamoeba spp.: In vitro culture, light and ultrastructural characterization, species identification by immunofluorescence method with monoclonal antibodies, in vitro pharmacological studies; c) Microsporidia: ultrastructural (TEM), biomolecular (PCR), biochemical and immunological (SDS-PAGE, Immunoblotting) studies for species identification, use of advanced ultrastructural techniques ("freeze-etching", "deep-etching") in order to deepen the spore wall structure, to study the cytoskeletal function of actin and to define the mode of infection, in vitro pharmacological assays on some inhibitors of chitin-synthases.
| Original language | English |
|---|---|
| Pages (from-to) | 37-44 |
| Number of pages | 8 |
| Journal | Parassitologia |
| Volume | 43 |
| Issue number | SUPPL. 1 |
| Publication status | Published - 2001 |
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Keywords
- Acanthamoeba spp
- Advanced diagnostic protocols
- E. Histolytica/E. Dispar
- Microsporidia
ASJC Scopus subject areas
- Immunology and Microbiology(all)
Cite this
Protozoi patogeni per l'uomo : Protocolli di caratterizzazione differenziale di genere e di specie nell'ambito del complesso Entamoeba histolytica/Entamoeba dispar, Acanthamoeba spp., Microsporidi. / Scaglia, Massimo; Gatti, S.; Bruno, A.; Bernuzzi, A. M.; Cevini, C.; Maserati, R.
In: Parassitologia, Vol. 43, No. SUPPL. 1, 2001, p. 37-44.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protozoi patogeni per l'uomo
T2 - Protocolli di caratterizzazione differenziale di genere e di specie nell'ambito del complesso Entamoeba histolytica/Entamoeba dispar, Acanthamoeba spp., Microsporidi
AU - Scaglia, Massimo
AU - Gatti, S.
AU - Bruno, A.
AU - Bernuzzi, A. M.
AU - Cevini, C.
AU - Maserati, R.
PY - 2001
Y1 - 2001
N2 - The review summarizes the results in the main parasitological topics of our Lab: amoebic infections due to Entamoeba histolytica/Entamoeba dispar complex and to Acanthamoeba spp. respectively, and human infections caused by microsporidia. Different rapid and advanced techniques have been included in the standardized diagnostic protocols for each topic, and a critical comparison among them was made, in order to define the gold standard diagnostic method: a) E. histolytica/E. dispar: in vitro culture, zymodeme typization, biomolecular identification (PCR), immunoenzymatic assay (ELISA) for direct detection in stools of specific surface antigenic lectins; b) Acanthamoeba spp.: In vitro culture, light and ultrastructural characterization, species identification by immunofluorescence method with monoclonal antibodies, in vitro pharmacological studies; c) Microsporidia: ultrastructural (TEM), biomolecular (PCR), biochemical and immunological (SDS-PAGE, Immunoblotting) studies for species identification, use of advanced ultrastructural techniques ("freeze-etching", "deep-etching") in order to deepen the spore wall structure, to study the cytoskeletal function of actin and to define the mode of infection, in vitro pharmacological assays on some inhibitors of chitin-synthases.
AB - The review summarizes the results in the main parasitological topics of our Lab: amoebic infections due to Entamoeba histolytica/Entamoeba dispar complex and to Acanthamoeba spp. respectively, and human infections caused by microsporidia. Different rapid and advanced techniques have been included in the standardized diagnostic protocols for each topic, and a critical comparison among them was made, in order to define the gold standard diagnostic method: a) E. histolytica/E. dispar: in vitro culture, zymodeme typization, biomolecular identification (PCR), immunoenzymatic assay (ELISA) for direct detection in stools of specific surface antigenic lectins; b) Acanthamoeba spp.: In vitro culture, light and ultrastructural characterization, species identification by immunofluorescence method with monoclonal antibodies, in vitro pharmacological studies; c) Microsporidia: ultrastructural (TEM), biomolecular (PCR), biochemical and immunological (SDS-PAGE, Immunoblotting) studies for species identification, use of advanced ultrastructural techniques ("freeze-etching", "deep-etching") in order to deepen the spore wall structure, to study the cytoskeletal function of actin and to define the mode of infection, in vitro pharmacological assays on some inhibitors of chitin-synthases.
KW - Acanthamoeba spp
KW - Advanced diagnostic protocols
KW - E. Histolytica/E. Dispar
KW - Microsporidia
UR - http://www.scopus.com/inward/record.url?scp=2242493671&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2242493671&partnerID=8YFLogxK
M3 - Article
VL - 43
SP - 37
EP - 44
JO - Parassitologia
JF - Parassitologia
SN - 0048-2951
IS - SUPPL. 1
ER -