Proximity of TPR and NTRK1 rearranging loci in human thyrocytes

Emanuela Roccato, Paola Bressan, Guido Sabatella, Cristiano Rumio, Laura Vizzotto, Marco A. Pierotti, Angela Greco

Research output: Contribution to journalArticle

Abstract

Chromosomal rearrangements are frequently associated with cancer; the mechanisms underlying their cell-type specificity are poorly understood. Papillary thyroid carcinomas are marked by a high frequency of chromosome rearrangements involving the RET and NTRK1 tyrosine kiinase receptor genes and producing RET and TRK oncogenes. An explanation for the propensity of thyrocytes to undergo gene rearrangements has been recently proposed by Nikiforova and colleagues, who showed that the recombination between RET and H4 is favored by the loci proximity in interphase nuclei. We investigated whether the spatial proximity is a contributing factor also in the generation of the thyroid-specific TRK oncogenes. The distance between NTRK1 and its oncogenic partner TPR was determined by two-color fluorescence in situ hybridization and two-dimensional microscopy. A three-dimensional reconstruction of the data was also done. We show that the two loci in thyrocytes nuclei display a distance reduced with respect to peripheral blood lymphocytes, thus supporting the notion that spatial proximity of translocation-prone gene loci may favor gene rearrangements.

Original languageEnglish
Pages (from-to)2572-2576
Number of pages5
JournalCancer Research
Volume65
Issue number7
DOIs
Publication statusPublished - Apr 1 2005

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Gene Rearrangement
Oncogenes
Interphase
Fluorescence In Situ Hybridization
Genetic Recombination
Genes
Microscopy
Thyroid Gland
Color
Chromosomes
Lymphocytes
Neoplasms
Thyroid Epithelial Cells
tyrosine receptor
Papillary Thyroid cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Roccato, E., Bressan, P., Sabatella, G., Rumio, C., Vizzotto, L., Pierotti, M. A., & Greco, A. (2005). Proximity of TPR and NTRK1 rearranging loci in human thyrocytes. Cancer Research, 65(7), 2572-2576. https://doi.org/10.1158/0008-5472.CAN-04-4294

Proximity of TPR and NTRK1 rearranging loci in human thyrocytes. / Roccato, Emanuela; Bressan, Paola; Sabatella, Guido; Rumio, Cristiano; Vizzotto, Laura; Pierotti, Marco A.; Greco, Angela.

In: Cancer Research, Vol. 65, No. 7, 01.04.2005, p. 2572-2576.

Research output: Contribution to journalArticle

Roccato, E, Bressan, P, Sabatella, G, Rumio, C, Vizzotto, L, Pierotti, MA & Greco, A 2005, 'Proximity of TPR and NTRK1 rearranging loci in human thyrocytes', Cancer Research, vol. 65, no. 7, pp. 2572-2576. https://doi.org/10.1158/0008-5472.CAN-04-4294
Roccato E, Bressan P, Sabatella G, Rumio C, Vizzotto L, Pierotti MA et al. Proximity of TPR and NTRK1 rearranging loci in human thyrocytes. Cancer Research. 2005 Apr 1;65(7):2572-2576. https://doi.org/10.1158/0008-5472.CAN-04-4294
Roccato, Emanuela ; Bressan, Paola ; Sabatella, Guido ; Rumio, Cristiano ; Vizzotto, Laura ; Pierotti, Marco A. ; Greco, Angela. / Proximity of TPR and NTRK1 rearranging loci in human thyrocytes. In: Cancer Research. 2005 ; Vol. 65, No. 7. pp. 2572-2576.
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