PRRT2 Is a Key Component of the Ca2+-Dependent Neurotransmitter Release Machinery

Pierluigi Valente, Enrico Castroflorio, Pia Rossi, Manuela Fadda, Bruno Sterlini, Romina Ines Cervigni, Cosimo Prestigio, Silvia Giovedi, Franco Onofri, Elisa Mura, Fabrizia C. Guarnieri, Antonella Marte, Marta Orlando, Federico Zara, Anna Fassio, Flavia Valtorta, P. Baldelli, Anna Corradi, F. Benfenati

Research output: Contribution to journalArticlepeer-review

Abstract

Heterozygous mutations in proline-rich transmembrane protein 2 (PRRT2) underlie a group of paroxysmal disorders, including epilepsy, kinesigenic dyskinesia, and migraine. Most of the mutations lead to impaired PRRT2 expression, suggesting that loss of PRRT2 function may contribute to pathogenesis. We show that PRRT2 is enriched in presynaptic terminals and that its silencing decreases the number of synapses and increases the number of docked synaptic vesicles at rest. PRRT2-silenced neurons exhibit a severe impairment of synchronous release, attributable to a sharp decrease in release probability and Ca2+ sensitivity and associated with a marked increase of the asynchronous/synchronous release ratio. PRRT2 interacts with the synaptic proteins SNAP-25 and synaptotagmin 1/2. The results indicate that PRRT2 is intimately connected with the Ca2+-sensing machinery and that it plays an important role in the final steps of neurotransmitter release.

Original languageEnglish
Pages (from-to)117-131
Number of pages15
JournalCell Reports
Volume15
Issue number1
DOIs
Publication statusPublished - Apr 5 2016

Keywords

  • Knockdown
  • PRRT2
  • Release probability
  • Synaptic transmission
  • Synaptotagmin
  • Synchronous and asynchronous release

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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