TY - JOUR
T1 - Prune cAMP phosphodiesterase binds nm23-H1 and promotes cancer metastasis
AU - D'Angelo, Anna
AU - Garzia, Livia
AU - André, Alessandra
AU - Carotenuto, Pietro
AU - Aglio, Veruska
AU - Guardiola, Ombretta
AU - Arrigoni, Gianluigi
AU - Cossu, Antonio
AU - Palmieri, Giuseppe
AU - Aravind, L.
AU - Zollo, Massimo
PY - 2004/2
Y1 - 2004/2
N2 - We identify a new enzymatic activity underlying metastasis in breast cancer and describe its susceptibility to therapeutic inhibition. We show that human prune (h-prune), a phosphoesterase DHH family appertaining protein, has a hitherto unrecognized cyclic nucleotide phosphodiesterase activity effectively suppressed by dipyridamole, a phosphodiesterase inhibitor. H-prune physically interacts with nm23-H1, a metastasis suppressor gene. The h-prune PDE activity, suppressed by dipyridamole and enhanced by the interaction with nm23-H1, stimulates cellular motility and metastasis processes. Out of 59 metastatic breast cancer cases analyzed, 22 (37%) were found to overexpress h-prune, evidence that this novel enzymatic activity is involved in promoting cancer metastasis.
AB - We identify a new enzymatic activity underlying metastasis in breast cancer and describe its susceptibility to therapeutic inhibition. We show that human prune (h-prune), a phosphoesterase DHH family appertaining protein, has a hitherto unrecognized cyclic nucleotide phosphodiesterase activity effectively suppressed by dipyridamole, a phosphodiesterase inhibitor. H-prune physically interacts with nm23-H1, a metastasis suppressor gene. The h-prune PDE activity, suppressed by dipyridamole and enhanced by the interaction with nm23-H1, stimulates cellular motility and metastasis processes. Out of 59 metastatic breast cancer cases analyzed, 22 (37%) were found to overexpress h-prune, evidence that this novel enzymatic activity is involved in promoting cancer metastasis.
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U2 - 10.1016/S1535-6108(04)00021-2
DO - 10.1016/S1535-6108(04)00021-2
M3 - Article
C2 - 14998490
AN - SCOPUS:10744220955
VL - 5
SP - 137
EP - 149
JO - Cancer Cell
JF - Cancer Cell
SN - 1535-6108
IS - 2
ER -