Pseudo-outbreak of Mycobacterium gordonae in a teaching hospital: Importance of strictlyfollowing decontamination procedures and emerging issues concerning sterilization

Laura Scorzolini, Fabio Mengoni, Claudio M. Mastroianni, Rossella Baldan, Daniela M. Cirillo, Maria De Giusti, Lucia Marinelli, Alessia Cottarelli, Lanfranco Fattorini, Vincenzo Vullo

Research output: Contribution to journalArticle

Abstract

Aim of this study was to investigate a pseudo-outbreak of Mycobacterium gordonae analyzing isolates detected from clinical and environmental samples. Mycobacterium gordonae was detected in 7 out of 497 broncho-Alveolar lavage (BAL) samples after bronchoscopy procedure in patients admitted to a teaching hospital between January and April 2013. During this pseudo-outbreak clinical, epidemiological, environmental and molecular investigations were performed. None of the patients met the criteria for non-Tuberculous mycobacterial (NTM) lung disease and were treated for M. gordonae lung disease. Environmental investigation revealed M. gordonae in 3 samples: in tap water and in the water supply channel of the washer disinfector. All the isolates were subjected to genotyping by pulsed-field gel electrophoresis (PFGE). The PFGE revealed that only patients' isolates presented the same band pattern but no correlation with the environmental strain was detected. Surveillance of the outbreak and the strict adherence to the reprocessing procedure and its supplies resulted afterwards in no detection of M. gordonae in clinical respiratory samples. Clinical surveillance of patients was crucial to establish the start of NTM treatment. Regular screening of tap water and endoscopic equipment should be adopted to compare the clinical strains with the environmental ones when an outbreak occurs.

Original languageEnglish
Pages (from-to)35-44
Number of pages10
JournalNew Microbiologica
Volume39
Issue number1
Publication statusPublished - Jan 1 2016

Keywords

  • Broncoschopy
  • Mycobacterium gordonae
  • Pseudo-outbreak
  • Pulsed-field gel electrophoresis

ASJC Scopus subject areas

  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Pseudo-outbreak of Mycobacterium gordonae in a teaching hospital: Importance of strictlyfollowing decontamination procedures and emerging issues concerning sterilization'. Together they form a unique fingerprint.

  • Cite this

    Scorzolini, L., Mengoni, F., Mastroianni, C. M., Baldan, R., Cirillo, D. M., De Giusti, M., Marinelli, L., Cottarelli, A., Fattorini, L., & Vullo, V. (2016). Pseudo-outbreak of Mycobacterium gordonae in a teaching hospital: Importance of strictlyfollowing decontamination procedures and emerging issues concerning sterilization. New Microbiologica, 39(1), 35-44.