Pseudomonas aeruginosa bloodstream infections: Risk factors and treatment outcome related to expression of the PER-I extended-spectrum beta-lactamase

Background: Bloodstream infection (BSI) due to Pseudomonas aeruginosa (Pa) has relevant clinical impact especially in relation to drug resistance determinants. The PER-I extended-spectrum beta-lactamase (ESBL) is a common enzyme conferring high-level resistance to anti-pseudomonal cephalosporins. Risk factors and treatment outcome of BSI episodes caused by PER-I-positive Pa (PER-I-Pa) strains were compared to those caused by ESBL-negative Pa isolates (ESBL-N-Pa). Methods: Twenty-six BSI cases due to ceftazidime-resistant Pa strains have been investigated. MIC values of anti-pseudomonal drugs were determined by the Etest method (AB Biodisk, Solna, Sweden). The double-disk synergy test was used to detect ESBL production. PCR amplification and DNA sequencing were used to characterize ESBL types. Clinical records of BSI-patients were examined retrospectively. Demographic data, underlying diseases (McCabe-Jackson classification and Charlson weighted index), risk factors, antimicrobial therapy, and treatment outcome were evaluated in cases due to ESBL-positive and cases due to ESBL-N-Pa isolates. Unpaired Student's t-test, Mann-Whitney U-test, Fisher's exact test and the χ² test were used for statistical analysis. Results: Nine Pa isolates expressed the PER-I ESBL; the remaining 17 isolates did not produce ESBLs. Severe sepsis (P=0.03), bladder and intravascular catheters (both P=0.01), immunosuppressive therapy (P=0.04), and mechanical ventilation (P=0.03) were significantly associated with BSI due to PER-I-Pa. Empirical treatment (P= 0.02) and treatment after ID/AST (P