pSTAT1, pSTAT3, and T-bet expression in peripheral blood mononuclear cells from relapsing-remitting multiple sclerosis patients correlates with disease activity

Giovanni Frisullo, Francesco Angelucci, Marcella Caggiula, Viviana Nociti, Raffaele Iorio, Agata Katia Patanella, Cristina Sancricca, Massimiliano Mirabella, Pietro Attilio Tonali, Anna Paola Batocchi

Research output: Contribution to journalArticle

Abstract

Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system, and it is considered to be a T helper 1 (Th1) cell-mediated autoimmune disease. T-bet has been identified as a key transcription factor for the development of Th1 cells and the induction of interferon (IFN)-γ production. T-bet is induced during T-cell activation by the IFN-γ signal transducer and activator of transcription (STAT)-1 signalling pathway. In this study we found an up-regulation of T-bet and pSTAT1 in peripheral blood CD4+ and CD8+ T cells and monocytes from relapsing-remitting MS patients in relapse compared with patients in remission and with healthy subjects. The increased expression of pSTAT1 strongly correlated with T-bet expression in CD4+ and CD8+ cells and monocytes from patients in relapse and was associated with an increased production of IFN-γ by peripheral blood mononuclear cells (PBMCs). pSTAT3 was also up-regulated in CD4+ and CD8+ cells and monocytes from patients in relapse and was associated with an increased production of interleukin (IL)-10 but not of IL-6. pSTAT1, pSTAT3, and T-bet expression strongly correlated with Gd-DTPA-enhanced lesions on brain and spinal cord magnetic resonance imaging. Our data show for the first time that there is an up-regulation of type 1 immunity-correlated transcription factors such as STAT1 and T-bet in peripheral blood subpopulations of MS patients in the active phase of disease. The evaluation of T-bet and pSTAT1 expression in peripheral blood CD4+, CD8+ T cells and monocytes could be used as a marker of disease activity in relapsing-remitting MS.

Original languageEnglish
Pages (from-to)1027-1036
Number of pages10
JournalJournal of Neuroscience Research
Volume84
Issue number5
DOIs
Publication statusPublished - Oct 2006

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Keywords

  • IFN-γ
  • IL-10
  • IL-6
  • Transcription factors
  • Type 1 immunity

ASJC Scopus subject areas

  • Neuroscience(all)

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