PTC is a novel rearranged form of the ret proto-oncogene and is frequently detected in vivo in human thyroid papillary carcinomas

Michele Grieco, Massimo Santoro, Maria Teresa Berlingieri, Rosa Marina Melillo, Rosangela Donghi, Italia Bongarzone, Marco A. Pierotti, Giuseppe Della Ports, Alfredo Fusco, Giancarlo Vecchiot

Research output: Contribution to journalArticlepeer-review

Abstract

We recently detected a novel activated oncogene by transfection analysis on NIH 3T3 cells in five out of 20 primary human thyroid papillary carcinomas and in the available lymph node metastases. We designated this transforming gene FTC (for papillary thyroid carcinoma). Here we describe the molecular cloning and sequencing of the gene. The new oncogene resulted from the rearrangement of an unknown amino,terminal sequence to the tyrosine kinase domain of the ret proto-oncogene. This gene rearrangement was detected in all of the transfectants and In all of the original tumor DNAs, but not in normal DNA of the same patients, thus indicating that this genetic lesion occurred in vivo and is specific to somatic tumors. Moreover, the transcript coded for by the fused gene was detected in an additional FTC-positive human papillary carcinoma for which mRNA was available.

Original languageEnglish
Pages (from-to)557-563
Number of pages7
JournalCell
Volume60
Issue number4
DOIs
Publication statusPublished - Feb 23 1990

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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