pUL25 immunolocalization in human cytomegalovirus-infected and gene-transfected cells

N. Zini, S. Santi, M. Riccio, M. P. Landini, M. C. Battista, N. M. Maraldi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

By means of confocal and electron microscope immunocytochemistry we have studied the localization of a recently described structural protein (pUL25) of human cytomegalovirus, in both infected cells and in cells transiently transfected with UL25. pUL25 localization in infected cells was observed in typical cytoplasmic structures characterized by a very electrondense texture previously reported to accumulate other tegument proteins. At the virion level pUL25 seems to localize at the interface between the tegument and the capsid of both intracytoplasmic and extracellular virions. In UL-25-transfected cells, pUL25 has been found in characteristic para-crystalline cytoplasmic aggregates, suggesting its intrinsic ability to aggregate in a regular subunit pattern.

Original languageEnglish
Pages (from-to)795-803
Number of pages9
JournalArchives of Virology
Volume145
Issue number4
Publication statusPublished - 2000

Fingerprint

Cytomegalovirus
Virion
Genes
Cytoplasmic Structures
Capsid
Proteins
Immunohistochemistry
Electrons

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Zini, N., Santi, S., Riccio, M., Landini, M. P., Battista, M. C., & Maraldi, N. M. (2000). pUL25 immunolocalization in human cytomegalovirus-infected and gene-transfected cells. Archives of Virology, 145(4), 795-803.

pUL25 immunolocalization in human cytomegalovirus-infected and gene-transfected cells. / Zini, N.; Santi, S.; Riccio, M.; Landini, M. P.; Battista, M. C.; Maraldi, N. M.

In: Archives of Virology, Vol. 145, No. 4, 2000, p. 795-803.

Research output: Contribution to journalArticle

Zini, N, Santi, S, Riccio, M, Landini, MP, Battista, MC & Maraldi, NM 2000, 'pUL25 immunolocalization in human cytomegalovirus-infected and gene-transfected cells', Archives of Virology, vol. 145, no. 4, pp. 795-803.
Zini N, Santi S, Riccio M, Landini MP, Battista MC, Maraldi NM. pUL25 immunolocalization in human cytomegalovirus-infected and gene-transfected cells. Archives of Virology. 2000;145(4):795-803.
Zini, N. ; Santi, S. ; Riccio, M. ; Landini, M. P. ; Battista, M. C. ; Maraldi, N. M. / pUL25 immunolocalization in human cytomegalovirus-infected and gene-transfected cells. In: Archives of Virology. 2000 ; Vol. 145, No. 4. pp. 795-803.
@article{be41cc203ec243f8ae240b37647ed0e5,
title = "pUL25 immunolocalization in human cytomegalovirus-infected and gene-transfected cells",
abstract = "By means of confocal and electron microscope immunocytochemistry we have studied the localization of a recently described structural protein (pUL25) of human cytomegalovirus, in both infected cells and in cells transiently transfected with UL25. pUL25 localization in infected cells was observed in typical cytoplasmic structures characterized by a very electrondense texture previously reported to accumulate other tegument proteins. At the virion level pUL25 seems to localize at the interface between the tegument and the capsid of both intracytoplasmic and extracellular virions. In UL-25-transfected cells, pUL25 has been found in characteristic para-crystalline cytoplasmic aggregates, suggesting its intrinsic ability to aggregate in a regular subunit pattern.",
author = "N. Zini and S. Santi and M. Riccio and Landini, {M. P.} and Battista, {M. C.} and Maraldi, {N. M.}",
year = "2000",
language = "English",
volume = "145",
pages = "795--803",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer Wien",
number = "4",

}

TY - JOUR

T1 - pUL25 immunolocalization in human cytomegalovirus-infected and gene-transfected cells

AU - Zini, N.

AU - Santi, S.

AU - Riccio, M.

AU - Landini, M. P.

AU - Battista, M. C.

AU - Maraldi, N. M.

PY - 2000

Y1 - 2000

N2 - By means of confocal and electron microscope immunocytochemistry we have studied the localization of a recently described structural protein (pUL25) of human cytomegalovirus, in both infected cells and in cells transiently transfected with UL25. pUL25 localization in infected cells was observed in typical cytoplasmic structures characterized by a very electrondense texture previously reported to accumulate other tegument proteins. At the virion level pUL25 seems to localize at the interface between the tegument and the capsid of both intracytoplasmic and extracellular virions. In UL-25-transfected cells, pUL25 has been found in characteristic para-crystalline cytoplasmic aggregates, suggesting its intrinsic ability to aggregate in a regular subunit pattern.

AB - By means of confocal and electron microscope immunocytochemistry we have studied the localization of a recently described structural protein (pUL25) of human cytomegalovirus, in both infected cells and in cells transiently transfected with UL25. pUL25 localization in infected cells was observed in typical cytoplasmic structures characterized by a very electrondense texture previously reported to accumulate other tegument proteins. At the virion level pUL25 seems to localize at the interface between the tegument and the capsid of both intracytoplasmic and extracellular virions. In UL-25-transfected cells, pUL25 has been found in characteristic para-crystalline cytoplasmic aggregates, suggesting its intrinsic ability to aggregate in a regular subunit pattern.

UR - http://www.scopus.com/inward/record.url?scp=0034063455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034063455&partnerID=8YFLogxK

M3 - Article

VL - 145

SP - 795

EP - 803

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 4

ER -