pUL25 immunolocalization in human cytomegalovirus-infected and gene-transfected cells

N. Zini, S. Santi, M. Riccio, M. P. Landini, M. C. Battista, N. M. Maraldi

Research output: Contribution to journalArticlepeer-review


By means of confocal and electron microscope immunocytochemistry we have studied the localization of a recently described structural protein (pUL25) of human cytomegalovirus, in both infected cells and in cells transiently transfected with UL25. pUL25 localization in infected cells was observed in typical cytoplasmic structures characterized by a very electrondense texture previously reported to accumulate other tegument proteins. At the virion level pUL25 seems to localize at the interface between the tegument and the capsid of both intracytoplasmic and extracellular virions. In UL-25-transfected cells, pUL25 has been found in characteristic para-crystalline cytoplasmic aggregates, suggesting its intrinsic ability to aggregate in a regular subunit pattern.

Original languageEnglish
Pages (from-to)795-803
Number of pages9
JournalArchives of Virology
Issue number4
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

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