Purification and biochemical characterization of IMP-13 metallo-β-lactamase

Gisela Santella, Jean Denis Docquier, Gabriel Gutkind, Gian Maria Rossolini, Marcela Radice

Research output: Contribution to journalArticlepeer-review

Abstract

The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.

Original languageEnglish
Pages (from-to)399-401
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume55
Issue number1
DOIs
Publication statusPublished - Jan 2011

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

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