Purification and biochemical characterization of IMP-13 metallo-β-lactamase

Gisela Santella; Jean Denis Docquier; Gabriel Gutkind; Gian Maria Rossolini; Marcela Radice

doi:10.1128/AAC.00421-10

Purification and biochemical characterization of IMP-13 metallo-β-lactamase

Gisela Santella, Jean Denis Docquier, Gabriel Gutkind, Gian Maria Rossolini, Marcela Radice

Fondazione Don Carlo Gnocchi Onlus

Research output: Contribution to journal › Article › peer-review

Abstract

The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.

Original language	English
Pages (from-to)	399-401
Number of pages	3
Journal	Antimicrobial Agents and Chemotherapy
Volume	55
Issue number	1
DOIs	https://doi.org/10.1128/AAC.00421-10
Publication status	Published - Jan 2011

ASJC Scopus subject areas

Pharmacology (medical)
Pharmacology
Infectious Diseases

Access to Document

10.1128/AAC.00421-10

Fingerprint Dive into the research topics of 'Purification and biochemical characterization of IMP-13 metallo-β-lactamase'. Together they form a unique fingerprint.

Cite this

@article{52c7af4a1c67469eb12c89b970c19838,

title = "Purification and biochemical characterization of IMP-13 metallo-β-lactamase",

abstract = "The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.",

author = "Gisela Santella and Docquier, {Jean Denis} and Gabriel Gutkind and Rossolini, {Gian Maria} and Marcela Radice",

year = "2011",

month = jan,

doi = "10.1128/AAC.00421-10",

language = "English",

volume = "55",

pages = "399--401",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "1",

}

TY - JOUR

T1 - Purification and biochemical characterization of IMP-13 metallo-β-lactamase

AU - Santella, Gisela

AU - Docquier, Jean Denis

AU - Gutkind, Gabriel

AU - Rossolini, Gian Maria

AU - Radice, Marcela

PY - 2011/1

Y1 - 2011/1

N2 - The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.

AB - The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.

UR - http://www.scopus.com/inward/record.url?scp=78650636278&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650636278&partnerID=8YFLogxK

U2 - 10.1128/AAC.00421-10

DO - 10.1128/AAC.00421-10

M3 - Article

C2 - 20974864

AN - SCOPUS:78650636278

VL - 55

SP - 399

EP - 401

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 1

ER -