Purification and biochemical characterization of IMP-13 metallo-β-lactamase

Gisela Santella, Jean Denis Docquier, Gabriel Gutkind, Gian Maria Rossolini, Marcela Radice

Research output: Contribution to journalArticle

Abstract

The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.

Original languageEnglish
Pages (from-to)399-401
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume55
Issue number1
DOIs
Publication statusPublished - Jan 2011

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Inosine Monophosphate
meropenem
Ticarcillin
Piperacillin
Imipenem
Chromatography
Escherichia coli
Enzymes

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Santella, G., Docquier, J. D., Gutkind, G., Rossolini, G. M., & Radice, M. (2011). Purification and biochemical characterization of IMP-13 metallo-β-lactamase. Antimicrobial Agents and Chemotherapy, 55(1), 399-401. https://doi.org/10.1128/AAC.00421-10

Purification and biochemical characterization of IMP-13 metallo-β-lactamase. / Santella, Gisela; Docquier, Jean Denis; Gutkind, Gabriel; Rossolini, Gian Maria; Radice, Marcela.

In: Antimicrobial Agents and Chemotherapy, Vol. 55, No. 1, 01.2011, p. 399-401.

Research output: Contribution to journalArticle

Santella, G, Docquier, JD, Gutkind, G, Rossolini, GM & Radice, M 2011, 'Purification and biochemical characterization of IMP-13 metallo-β-lactamase', Antimicrobial Agents and Chemotherapy, vol. 55, no. 1, pp. 399-401. https://doi.org/10.1128/AAC.00421-10
Santella, Gisela ; Docquier, Jean Denis ; Gutkind, Gabriel ; Rossolini, Gian Maria ; Radice, Marcela. / Purification and biochemical characterization of IMP-13 metallo-β-lactamase. In: Antimicrobial Agents and Chemotherapy. 2011 ; Vol. 55, No. 1. pp. 399-401.
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