Abstract
The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.
| Original language | English |
|---|---|
| Pages (from-to) | 399-401 |
| Number of pages | 3 |
| Journal | Antimicrobial Agents and Chemotherapy |
| Volume | 55 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2011 |
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ASJC Scopus subject areas
- Pharmacology (medical)
- Pharmacology
- Infectious Diseases
Cite this
Purification and biochemical characterization of IMP-13 metallo-β-lactamase. / Santella, Gisela; Docquier, Jean Denis; Gutkind, Gabriel; Rossolini, Gian Maria; Radice, Marcela.
In: Antimicrobial Agents and Chemotherapy, Vol. 55, No. 1, 01.2011, p. 399-401.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Purification and biochemical characterization of IMP-13 metallo-β-lactamase
AU - Santella, Gisela
AU - Docquier, Jean Denis
AU - Gutkind, Gabriel
AU - Rossolini, Gian Maria
AU - Radice, Marcela
PY - 2011/1
Y1 - 2011/1
N2 - The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.
AB - The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.
UR - http://www.scopus.com/inward/record.url?scp=78650636278&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650636278&partnerID=8YFLogxK
U2 - 10.1128/AAC.00421-10
DO - 10.1128/AAC.00421-10
M3 - Article
C2 - 20974864
AN - SCOPUS:78650636278
VL - 55
SP - 399
EP - 401
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
SN - 0066-4804
IS - 1
ER -