TY - JOUR
T1 - Purification and biochemical characterization of IMP-13 metallo-β-lactamase
AU - Santella, Gisela
AU - Docquier, Jean Denis
AU - Gutkind, Gabriel
AU - Rossolini, Gian Maria
AU - Radice, Marcela
PY - 2011/1
Y1 - 2011/1
N2 - The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.
AB - The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.
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U2 - 10.1128/AAC.00421-10
DO - 10.1128/AAC.00421-10
M3 - Article
C2 - 20974864
AN - SCOPUS:78650636278
VL - 55
SP - 399
EP - 401
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
SN - 0066-4804
IS - 1
ER -