Purification and characterization of Ag,Zn-superoxide dismutase from Saccharomyces cerevisiae exposed to silver

Maria R. Ciriolo, Patrizia Civitareale, M. Teresa Carrì, Angelo De Martino, Francesca Galiazzo, Giuseppe Rotilio

Research output: Contribution to journalArticle

Abstract

Cu,Zn-superoxide dismutase plays an important role in protecting cells from oxygen toxicity by catalyzing the dismutation of superoxide anion into hydrogen peroxide and oxygen. In Saccharomyces cerevisiae Cu,Zn-superoxide dismutase is coregulated with copper-thionein by copper via the transcription factor ACE 1. We demonstrate here that presence of AgNO3 in the culture medium leads to a five times increase of Cu,Zn-superoxide dismutase mRNA, with a concomitant six times decrease of the enzyme activity. Susceptibility of yeast to silver was apparently inversely related to Cu,Zn-superoxide dismutase activity. From silver-treated yeast a Cu,Zn-superoxide dismutase with impaired dismutase function was purified and was shown to contain silver, which was located to the copper site. These data suggest that Cu,Zn- superoxide dismutase may play an additional direct role in the defense of S. cerevisiae against metal stress by functioning as metal chelator.

Original languageEnglish
Pages (from-to)25783-25787
Number of pages5
JournalJournal of Biological Chemistry
Volume269
Issue number41
Publication statusPublished - Oct 14 1994

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Silver
Yeast
Superoxide Dismutase
Purification
Saccharomyces cerevisiae
Copper
Yeasts
Metals
Oxygen
Enzyme activity
Chelating Agents
Superoxides
Hydrogen Peroxide
Toxicity
Culture Media
Superoxide Dismutase-1
Transcription Factors
Messenger RNA
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Purification and characterization of Ag,Zn-superoxide dismutase from Saccharomyces cerevisiae exposed to silver. / Ciriolo, Maria R.; Civitareale, Patrizia; Carrì, M. Teresa; De Martino, Angelo; Galiazzo, Francesca; Rotilio, Giuseppe.

In: Journal of Biological Chemistry, Vol. 269, No. 41, 14.10.1994, p. 25783-25787.

Research output: Contribution to journalArticle

Ciriolo, Maria R. ; Civitareale, Patrizia ; Carrì, M. Teresa ; De Martino, Angelo ; Galiazzo, Francesca ; Rotilio, Giuseppe. / Purification and characterization of Ag,Zn-superoxide dismutase from Saccharomyces cerevisiae exposed to silver. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 41. pp. 25783-25787.
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