Purification and characterization of Ag,Zn-superoxide dismutase from Saccharomyces cerevisiae exposed to silver

Maria R. Ciriolo, Patrizia Civitareale, M. Teresa Carrì, Angelo De Martino, Francesca Galiazzo, Giuseppe Rotilio

Research output: Contribution to journalArticlepeer-review

Abstract

Cu,Zn-superoxide dismutase plays an important role in protecting cells from oxygen toxicity by catalyzing the dismutation of superoxide anion into hydrogen peroxide and oxygen. In Saccharomyces cerevisiae Cu,Zn-superoxide dismutase is coregulated with copper-thionein by copper via the transcription factor ACE 1. We demonstrate here that presence of AgNO3 in the culture medium leads to a five times increase of Cu,Zn-superoxide dismutase mRNA, with a concomitant six times decrease of the enzyme activity. Susceptibility of yeast to silver was apparently inversely related to Cu,Zn-superoxide dismutase activity. From silver-treated yeast a Cu,Zn-superoxide dismutase with impaired dismutase function was purified and was shown to contain silver, which was located to the copper site. These data suggest that Cu,Zn- superoxide dismutase may play an additional direct role in the defense of S. cerevisiae against metal stress by functioning as metal chelator.

Original languageEnglish
Pages (from-to)25783-25787
Number of pages5
JournalJournal of Biological Chemistry
Volume269
Issue number41
Publication statusPublished - Oct 14 1994

ASJC Scopus subject areas

  • Biochemistry

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